Cas9 Stable Cell Line    

Product Details

Cas9 stable cell lines are a valuable resource for researchers conducting gene editing experiments. These cell lines stably and consistently express Cas9 nuclease, providing a reliable platform for precise and targeted gene modification. Creative Biogene CRISPR/Cas9 Platform^CB offers customers a range of Cas9 stable cell lines that can be used in various fields, including genetic disease treatment, disease-related gene screening and detection, tumor therapy, and prevention of pathogenic microorganisms.

Background

The CRISPR/Cas9 knockout system is composed of a Cas9 protein with DNA cleavage activity and a gRNA that recognizes a specific target. This simplifies the construction of knockout vectors because target recognition can be achieved by editing only the gRNA in the system. In mammalian cell culture systems, most gene editing is accomplished by transient transfection and lentiviral transduction, which is effective for routine low-throughput applications. However, for other applications, it may be more effective to stably express one of the components, such as the CRISPR-Cas9 nuclease.

Applications

• sgRNA functional validation

It is important to note that there are differences in the efficiency of individual sgRNAs themselves. Prior to extensive screening efforts, it is necessary to determine which CRISPR sgRNAs are more efficient at genome editing. Researchers can assess the efficiency and specificity of Cas9-mediated genome editing by introducing sgRNAs into Cas9-stabilized cell lines.

• Gene knockout using lentiviral CRISPR

Cas9 stable cell lines are useful for gene knockout experiments using the lentiviral CRISPR system. Transduction of Cas9 stable cell lines with lentivirus expressing sgRNA enables efficient genome editing.

• sgRNA library screening

CRISPR sgRNA libraries are commonly used for drug target identification, validation, phenotypic analysis, and reporter assays. CRISPR induces permanent changes to the genetic code, allowing for complete knockout of all gene alleles, regardless of their localization in the nucleus or cytoplasm.

• Inducible genome editing

Cas9 stable cell lines enable inducible genome editing, providing researchers with greater flexibility and specificity in functional genomics studies. This approach is particularly useful for studying cellular processes and diseases.

Advantages

• A range of CRISPR-Cas9 stably expressing cell lines from human, mouse, and rat sources that cover various cancers, including lung, cervical, breast, and liver cancer.
• Our cell lines ensure stable Cas9 expression through site integration without any adverse effects on cells.
• Our Cas9 stable cell lines can be used in conjunction with sgRNA cloning, sgRNA library, and donor cloning services.

Creative Biogene CRISPR/Cas9 Platform^CB is committed to providing our customers with high-quality Cas9 stable cell line products. Our Cas9 stable cell lines offer researchers reliable and convenient tools for efficient gene editing using the CRISPR-Cas9 system. Please contact us for more information, including product availability, pricing and customization options.