Genome Editing in Cash Crops    

CRISPR/Cas9 Platform^CB provides one-stop genome editing service for cash crops, such as cotton, tomato, cucumber, grape and petunia. This service including sgRNA design, expression vector construction, transient validation in protoplast, genetic transformation and mutation detection. At CRISPR/Cas9 Platform^CB, all projects are done by well-trained staff under the guide of talented scientists. We will complete your project in a shot turnaround time with most competitive price.

Cash crop is also known as industrial crop, including vegetable crop, fruit crop, fibre crop, oil crop, sugar crop, flower crop and medicine crop. With the completeness of genomic sequencing project for cash crop like cotton and tomato, the emphasis of cash crop genomic research has focused on functional genome research. CRISPR/Cas9, as one of the most popular tools of reverse genetics, plays an essential role in the research of gene function and genetic melioration. Researchers utilize this technology to accomplish single and multiplex gene knockout, gene knockin, gene replacement, base editing, transcriptional regulation, epigenetic modification and high-throughput mutant library construction in plant.

Fig 1. Domestication of wild tomatoes through CRISPR/Cas9 technology (Li, et al. 2018)

CRISPR/Cas9 Platform^CB provides gene knockout, base editing and sgRNA library construction services in cash crops. By engineering resistance genes related to abiotic and biotic stresses, we offer customers modified crops with enhanced properties, such as high yields and nutrients, resistance to disease, pests, or drought tolerance. Based on our efficient editing vectors, fully-fledged transformation technology and precise mutation detection system, we will deliver at least 10 positive T1 lines with stable genetic traits and complete your project within 6-9 months.

Typical species

• Solanum lycopersicum

Tomato (Solanum lycopersicum) is the most important vegetable fruit, with annual production of 100 million tons. Conventional breeding to domesticate wild plants increases productivity but is often accompanied by decreased fitness and genetic diversity. Recently, a multiplex CRISPR/Cas9 strategy was used to edit genes related to day-length sensitivity, shoot architecture, flower and fruit production and nutrient content to accelerate improvement of tomato. CRISPR/Cas9 Platform^CB develops a multiplex genome editing system to simultaneously knockout 8 genes at most. In addition, we also provide mutant library construction service for research of functional genomics and genetic improvement.

Applications

• Research of gene function
• Crop breeding and improvement
• Construction of mutant library
• Transcriptional regulation
• Epigenetic modification

For further information, please feel free to contact us.