CRISPRi Cell Line    

CRISPRi expands the potential applications of CRISPR gene editing technology and offers new tools for gene regulation research. Creative Biogene CRISPR/Cas9 Platform^CB provides researchers worldwide with a variety of highly active CRISPRi cell lines that effectively inhibit transcription. Our top-notch cell line products allow for precise and reversible gene inhibition, providing valuable insights into gene function and regulatory mechanisms.

Introduction of CRISPRi

The CRISPRi system is based on CRISPR/Cas9, which can specifically inhibit the transcription of target genes rather than knockout the genes to achieve regulation. CRISPRi can knock out target genes at different levels and inhibit multiple genes simultaneously with reversible effects. The technology uses dCas9 as a synthetic transcription factor, which is a mutant Cas9 protein that lacks nuclease cleavage activity and produces programmable RNA-guided DNA-binding proteins in human cells. dCas9 is associated with transcriptional repressors, such as KRAB (Kruppel-associated box). dCas9-KRAB fusion proteins bind to the transcription start site (TSS) of target genes under the guidance of gRNA, blocking RNA polymerase and transcript elongation, and silencing the expression of target genes.

Fig. 1 Schematic diagram of the CRISPRi system. (Kampmann, M., 2018)

Overview of Our CRISPRi Cell Line

Creative Biogene CRISPR/Cas9 Platform^CB utilizes the potential of CRISPRi technology to develop a range of ready-to-use CRISPRi cell lines. To construct CRISPRi cell lines, we integrate dCas9 (catalytically inactivated Cas9) proteins into stable host cell lines to ensure consistent and heritable gene regulation. We select commonly used and well-characterized host cell lines such as blood lineage cells, cancer cell lines, stem cells (iPSC) or other relevant cell lines to meet the research demands of different clients. We also amplify and screen infected cells, identify monoclonal strains by PCR and sequencing, and screen for successfully edited cell lines to ensure that we provide our customers with high-quality and active CRISPRi cell line products.

Application of Our CRISPRi Cell Lines

• Functional genomics
• Disease modeling
• Drug target identification and validation
• Pathway analysis and functional screening
• Epigenetic studies
• Synthetic biology and metabolic engineering

Why Choose Us?

• High-quality CRISPRi cell line products created using the latest CRISPRi technology.
• Our CRISPRi cell lines undergo stringent quality control, are highly active and robustly inhibit transcription.
• CRISPRi cell line products derived from various host cell lines provide multiple options for seamless integration into customers' existing experimental systems.

Creative Biogene CRISPR/Cas9 Platform^CB is dedicated to providing our customers with the CRISPRi cell line products they need to advance their research and make breakthrough discoveries in the field of gene regulation. Please contact us for more information, including product availability, pricing and customization options.