Genome Editing in Grain Crops    

CRISPR/Cas9 Platform^CB provides clients with professional and comprehensive service of crop genome editing. Our serious scientists and skilled staff have rich theoretical knowledge and practical experience in plant genome editing. We edit various crops in the past decade, including rice, wheat, maize, soybean, barley, sorghum, millet and potato. With this remarkable genome editing system, we will bring plant breeders and researchers high-quality services.

Crops provide food, feed, fuel, and other consumable resources for human life, thereby contributing enormously to society. To increase agricultural productivity and accelerate sustainable agricultural development, there is an urgent need for innovation in crop breeding technology. Cross breeding, mutation breeding, and transgenic breeding are currently the main methodologies for crop improvement, but such time-consuming, laborious and untargeted breeding programs cannot keep pace with the demands for increased crop production. In term of the development of CRISPR/Cas9 system, genetic breeding and improvement of crops become easy, fast and low-cost.

Fig 1. Agronomic Traits improvement by precisely modifying the target gene.

Scientists of CRISPR/Cas9 Platform^CB have rich experience of using genome editing technology to improve the crop breeding methods, especially in rice and wheat. Most importantly, we can obtain edited crops of transgene-free, which are not limited by long and costly regulatory evaluation processes as well as by public concerns.

Typical species

• Oryza sativa

Rice (Oryza sativa) feeds more than 3.5 billion people worldwide. Premium quality rice grain is the demand of a growing population with better living standards. Currently, CRISPR/Cas9 has widely used in rice genome editing because of its simplicity, efficiency and versatility. In addition, the ability to target multiple genes of this system can facilitate pathway-level research of rice grain quality. With full-fledged editing system, CRISPR/Cas9 Platform^CB will deliver consistent, high-quality services.

• Triticum aestivum

Common wheat (Triticum aestivum) is a staple food for mankind. In the past few decades, both basic and applied research on common wheat has lagged behind other crop species due to its complex, polyploid genome and difficulties in genetic transformation. Recent breakthroughs in wheat genome editing permit a revolution in wheat biotechnology. Scientists use CRISPR/RNPs (ribonucleoproteins) and TECCDNA (transiently expressed CRISPR/Cas9 plasmids DNA) methods to produce modified wheat of transgene-free. CRISPR/Cas9 Platform^CB introduces these technologies and develops an efficient editing system in wheat.

Workflow of CRISPR/Cas9-mediated genome editing in crops

Item	Description	Time
SgRNA design	Sequence analysis and sgRNA design by bioinformatics tools(at least 3 sgRNAs).	~1 week
Expression vector construction	Multiple choices of vector system to achieve your research purposes.	2-4 weeks
Transient validation of protoplast	Transforming plasmid into protoplasts and extracting genome DNA for analysis of mutation frequency.	1-2 weeks
Genetic transformation	Agrobacterium/PEG mediated transformation and particle bombardment method.	8-16 weeks
Mutation detection	Various methods are available, PCR-RE assay, target site sequencing, amplicon sequencing.	2-4 weeks

Advantages

• Fast, efficient and stable editing system
• Short experimental period
• Years of experience in plant genome editing
• Multiple gene editing system

For further information, please feel free to contact us.