Gene Knockout Mouse Generation    

CRISPR/Cas9 Platform^CB offers gene knockout mouse generation services for our clients. With experienced scientists and excellent gene editing platform, our staff has successfully generated various gene KO mice by using CRISPR/Cas9. We provide a one-stop KO mouse generation service that covers from sgRNA design and construct, pronuclear microinjection to Founders genotyping and breeding. We are dedicated to utilize the latest technology to service our customers for achieving their goals.

Engineered nuclease-mediated genome editing, including CRISPR/Cas9 and TALENs, is the most commonly used method for gene editing. Since the CRISPR/Cas9 appears, it needs less time to generate a gene knockout mouse than ever. Based on the characters of CRISPR system, it has been broadly used all over the world. Now the CRISPR/Cas9 system is applied for generating gene knockout mice. The workflow for this service is as follows:

At CRISPR/Cas9 Platform^CB, the custom service begins with a free consultation. After customer submits the detailed requirement, our scientists will show the client feasible analysis result and an optional strategy to achieve the goal. Once customers confirm the order, our staff would begin to construct required vectors and prepare zygote for microinjection. Cas9 and sgRNA are directly injected to zygotes where gene editing will occur. Mice that develop from these embryos are genotyped or sequenced to determine if they are correctly engineered. Every steps are well-organized and arranged.

For more information, please feel free to contact us.