Custom Synthesized sgRNA    

sgRNA Synthesis

CRISPR/Cas9 technology has revolutionized modern biology, with gene editing now at the forefront of scientific research. At Creative Biogene, we specialize in delivering reliable and professional services aimed at advancing your research goals. Our chemically synthesized sgRNA products are meticulously designed for 100% sequence accuracy and efficient protein knockout capabilities. With added thiol and methoxy modifications at both ends, our sgRNA ensures enhanced stability and reduced off-target effects, providing scientists worldwide with trusted tools for successful gene editing experiments.

Advantages of Chemically Synthesized sgRNA

In vitro transcription and plasmid recombination expression are classic methods for producing sgRNA in the CRISPR-Cas9 gene editing system. However, these methods have practical limitations, including the time-consuming nature of synthesis and the difficulty in controlling the quality of sgRNA. Additionally, DNA or other contaminants may be introduced into cells. In contrast, chemically synthesized sgRNA offers several advantages. These factors make chemically synthesized sgRNA a more reliable choice for CRISPR editing. High-quality and controllable chemically synthesized sgRNA can optimize different CRISPR experimental conditions.

Quality Control and Product Specifications

Creative Biogene's sgRNA stands out for its exceptional purity, high efficiency, and reliable performance in gene editing applications.

• Length: Typically 100-150 nucleotides (customizable based on needs)
• Purity: >92%, verified by HPLC and PAGE to ensure high purity and low impurity levels.
• Endotoxin:<1 EU/µg
• Packaging: Various packaging options available according to customer requirements
• Enhanced Stability: Modifications added to the 5' and 3' ends of the sgRNA
• LC-MS Detection: Molecular weight deviation less than 0.05%

Related Validation Services

We offer comprehensive validation services for chemically modified sgRNAs to ensure efficient, safe, and stable gene editing. Our services include evaluating genome editing efficiency in various cell lines, detecting off-target effects using deep sequencing, assessing cytotoxicity and stability during delivery, verifying gene function through knockout and repair experiments, and evaluating therapeutic efficacy in disease models. These validations help clients achieve high specificity and low off-target effects, promoting the broad application of CRISPR-Cas technology in biotechnology and therapeutic fields.

Background

Guide RNA (gRNA) is a part of the CRISPR-Cas9 system. A gRNA molecule consists of two components: a target-specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA). The gRNA guides the Cas protein complex to the corresponding site for cutting through base pairing between the 20-mer crRNA sequence and the target genomic sequence, determining the specificity of the editing site.

Single-guide RNA (sgRNA) represents a single-guide RNA that combines crRNA and tracrRNA into a single long RNA, usually around 100 nucleotides long, retaining the ability to cut double-stranded DNA. sgRNA can be expressed from a vector or chemically synthesized.

Application

1. Drug Development: Use sgRNA-based gene editing models for drug target validation, drug screening, and mechanism research.

2. Clinical Research: Provide efficient and safe gene editing tools for gene therapy and clinical research.

3. Agriculture And Plant Science: Using sgRNA for crop gene editing can improve crop disease resistance, yield, and nutritional value, thereby promoting the development of agricultural production.

4. Animal Model: Through sgRNA-mediated gene editing, accurate animal models are generated for disease research, helping scientists better understand the pathogenesis of human diseases and develop treatment options.

5. Synthetic Biology: In synthetic biology, sgRNA is used for genome reprogramming, designing and constructing new biological systems, and creating microorganisms or cells with specific functions for industrial biotechnology and environmental remediation.

6. Biosensing and Diagnosis: Develop sgRNA-based biosensors to detect specific DNA or RNA sequences, thereby achieving early diagnosis and monitoring of diseases.

Choose Us

Why Choose Creative Biogene?

Creative Biogene offers comprehensive sgRNA customization services, from design to synthesis, ensuring the success of your gene editing experiments. Our extensive experience and advanced technology ensure that each sgRNA meets the highest standards. For more details on sgRNA types and design, please contact us. We are committed to providing the highest quality products and services for your gene editing experiments.