Base Editing Library Screening Service    

High-throughput studies of gene function can be achieved by using gene editing technology to introduce different types of base mutations in specific regions of genes and using them as the basis for functional screening. Creative Biogene CRISPR/Cas9 Platform^CB provides one-stop base editing library screening services. This powerful and precise targeted genomic modification method enables researchers to analyze specific nucleotide-level genetic changes.

Overview of Base Editing Library Screening Service

Gene mutation libraries are constructed using the CRISPR-Cas9 system to introduce mutations into a target region to disrupt gene expression, thereby identifying gene function based on phenotype. Base editing systems, which can introduce efficient single-base transitions in specific regions of genes, have been rapidly applied to breakthrough results in studies such as high-throughput screening of gene functions related to DNA damage response or cancer. Creative Biogene CRISPR/Cas9 Platform^CB provides various types of base editing tools, and we can select the most suitable base editing system according to the research needs of our customers, in order to realize efficient and precise gene editing and gene function analysis. With our efficient base editing library screening service, we can help our clients to make progress in gene function research, disease modeling, and preclinical research.

Fig. 1 CRISPR DNA base editing tools. (Kantor, A. et al., 2020)

Our Base Editing Library Screening Service Process

Our base editing library screening service helps researchers conduct large-scale gene function studies to reveal biological processes or disease mechanisms by combining cutting-edge base editing tools with CRISPR screening technology. Below is our specific service process:

• Library construction

Our experienced experts design and construct CRISPR libraries based on the specific genomic regions of interest to our clients. This includes selecting the specific genomic region of interest and designing a series of guide RNAs (gRNAs) for base editing and subsequent screening. Moreover, we offer a variety of base editing components (e.g., CBE, ABE, or prime editors) to construct gRNA libraries to introduce specific modifications in target genes. Our efficient base editing strategy directly alters specific nucleotides without causing DNA breaks, reducing the incidence of off-target effects and improving the accuracy of the screening process.

• Gene editing and selection

We utilize efficient viral or plasmid delivery systems to introduce constructed base editing libraries into cells for desired modifications. Our experts can screen or enrich the cells to sort out the specific phenotype of interest, depending on the specific purpose of the screening.

• Functional screening

Edited cells were analyzed using fluorescence-based assays, cell sorting, or high-throughput sequencing techniques, among others, to identify specific base changes.

• Data analysis and interpretation

Our scientists utilize bioinformatics tools and statistical methods to correlate the screening results with the original CRIPR libraries, providing our clients with in-depth functional insights into their genes and delivering complete analysis reports.

Advantages of Our Base Editing Library Screening Service

• Customized strategies enable simultaneous multiple editing of multiple genomic loci to efficiently screen large libraries, saving time and resources.
• A variety of efficient base editors are available to minimize off-target effects.
• Advanced bioinformatics tools combined with data visualization techniques facilitated the interpretation of screening results.

Creative Biogene CRISPR/Cas9 Platform^CB is staffed by a team of experienced scientists with deep expertise in base editing technologies and CRISPR screening. We are committed to providing you with comprehensive base editing library screening services designed to help you achieve efficient and precise gene editing and bring progress to your research. Contact us today to learn how our services can support your scientific endeavors.