Custom CRISPR/Cas9 HDR Vectors    

Homology repair (HDR) is a natural DNA repair mechanism induced by DNA double-strand breaks (DSB). The CRISPR/Cas9 system generates DSB on the chromosome, resulting in DNA repair. In the presence of donor DNA, homologous directed repair (HDR) occurs and replaces the genetic information (insertion, removal or substitution) in the chromosome with new information from the donor DNA.

CRISPR/Cas9 Platform^CB provides a competitive price for synthesizing donor DNA sequences, cloning them into your chosen vector and performing sequence verification, regardless of the size or complexity of the vector. Our expertise and experience in highly reliable CRISPR technology applications make us a valuable partner for researchers worldwide.

The strategy of HDR repair with donor vector is described as below

Workflow of custom CRISOR/Cas9 HDR vectors

• Plan experiment
  ➢ Choose the right type of donor templates  
  ➢ Design an assay to efficiently identify HDR events
• Design sequences
  ➢ dsDNA donor template optimal for HDR (prevent the HDR template from rebuilding the PAM position near the insertion and avoid unnecessary re-cutting in the same position after the repair.)
• prepare reagents
• Gene assembly and cloning
  ➢ Choose the right type of vectors according to your needs
• Quality control
  ➢ Verification by PCR and sequencing
• Delivery
  ➢ 2-5 μg lyophilized plasmids containing sequence verified synthetic construct
  ➢ Sequence alignment data and alignment
  ➢ Separate synthetic gene sequences and sequence files in a vector library

You only need to provide

• Species
• NCBI gene ID or gene symbol
• Genomic locus that needs to be changed
• Sites marked with mKate2, EGFP, etc. or custom sequence insertion

Applications

• Insert reporter genes or fluorescent markers
• Fully customized inserts at any desired genomic locus
• Introduce base pair changes for mutagenesis or disease correction

Expected turnaround time

2-3 weeks
CRISPR/Cas9 Platform^CB is dedicated to providing professional CRISPR/Cas9 gene editing services and products for biotechnology, biomedical research, and pharmaceutical research. We provide you with custom HDR vectors service from strategic design to final stable and efficient HDR vectors, and keep in touch with you to report on project progress. We guarantee the quality of our products and deliver them at the most reasonable price in the shortest turnaround time. We will meet your specific requirements to protect your scientific investment. If you have any question, please feel free to contact us.