Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Comprehensive sgRNA Large-Scale Production Services 
sgRNA Production
Creative Biogene offers three purification methods for sgRNA synthesis services: RUO (Research Use Only), HPLC (High-Performance Liquid Chromatography), and GMP grade, to meet the wide-ranging needs of scientific research, preclinical validation, and clinical treatments. We provide an AI algorithm platform for sgRNA design, helping researchers quickly and efficiently design suitable sgRNA sequences for their studies.
Additionally, we offer chemically modified sgRNA to prevent immune responses and exonuclease degradation in primary cells, enhancing the stability and durability of gRNA within cells.
Figure 1. Creative Biogene provides Comprehensive sgRNA Supply in Biomedical Research and Clinical Trials.
sgRNA Production and Delivery Process
When you are ready to order GMP-grade sgRNA, Creative Biogene's custom and production teams will follow up on your project requirements, ensuring the entire project process and customer satisfaction. Our procurement process is as follows:
- Order Confirmation: After the customer places an order, we will confirm all technical details and project requirements with you.
- Design Confirmation: Ensure the sgRNA design and production meet your expectations and standards.
- Technical Approval: Our technical team will conduct a comprehensive evaluation and approval of the project to ensure all parameters meet production requirements.
- Production Initiation: We will start the sgRNA production process using TCRNA technology after approval.
- Quality Control: During production, strict LCMS quality monitoring is adopted to ensure the high quality and consistency of each batch of products.
- Product Transportation: After production is completed, we will ensure the products are delivered to your specified location safely and quickly, ensuring timely delivery.
Advantage
Advantages of Our sgRNA Large-Scale Production Services
Creative Biogene, as a leading gene-editing technology service provider, is committed to providing customers with first-class large sgRNA production services, featuring the following significant advantages:
- Fully Customized Synthesis: Provides high-quality RNA ranging from 90-164nt in length, supporting up to 10 types of chemical modifications.
- Strict Quality Control: Utilizes LCMS technology for quality monitoring, ensuring high reproducibility and stability of products. Our production processes comply with international standards, including GMP requirements from major regulatory agencies such as FDA, EMA, PMDA, and NMPA, ensuring product quality and safety.
- Wide Application: Supports extensive modifications suitable for efficient editing of different cell types and Cas enzymes.
- Professional Technical Support: Experienced research and technical support teams provide customized technical support and consulting services based on customer needs, ensuring smooth project progress and successful implementation.
- Quick Response and Delivery: Our procurement and production teams ensure a quick response to customer needs and timely and secure delivery of products to the specified location, supporting the urgent progress and timelines of customer projects.
Background
Advantages of Our CHO Cell Antibody Drug Production Service
Since its inception, CRISPR/Cas9 technology has rapidly evolved, becoming a hot topic in biological research. On November 16, 2023, the Medicines and Healthcare Products Regulatory Agency (MHRA) granted conditional marketing authorization for the gene therapy CASGEVY. This is the world's first approved CRISPR-based gene editing treatment. Additionally, over a dozen CRISPR gene editing therapies have entered clinical trial stages. In terms of disease applications, these therapies are expanding from treating genetic disorders to chronic diseases such as cardiovascular diseases and HIV infections, promising broader application prospects in the future.
Precision of CRISPR Technology with sgRNA
CRISPR technology relies on single guide RNA (sgRNA) to direct the Cas9 protein to the target DNA sequence, achieving precise gene editing. The sgRNA, with its 20nucleotide guide sequence, pairs with the target DNA sequence, ensuring Cas9 cuts the DNA at the correct position. This guiding function not only determines the specificity and efficiency of the CRISPR system but also allows optimization of the sgRNA sequence to reduce off-target effects, enhancing the accuracy of gene editing. Furthermore, the versatility of sgRNA enables it to perform gene knockouts, and, when combined with other CRISPR-associated proteins, to activate or inhibit genes and modify epigenetics, broadening its application in genomic operations.
Efficient Production Methods to Overcome Limitations
Traditional sgRNA synthesis methods include plasmid transfection and in vitro transcription; however, these methods often have unstable success rates and may cause cytotoxicity, limiting their large-scale production. The chemical synthesis of sgRNA effectively avoids these issues.
Contact Us
Comprehensive Support from Creative Biogene
Creative Biogene is proud to offer key sgRNA raw materials, customized quality control testing, and professional technical support for your base editing therapies, from R&D to clinical research stages. Our high-standard gene editing reagents ensure the successful application of downstream high-quality gene therapy products.
At Creative Biogene, we are committed to delivering steadfast support for your gene-editing endeavors and therapeutic innovations. Our mission is to expedite scientific breakthroughs and propel clinical trials forward, facilitating the swift advancement of gene and cell therapies that promise to enhance patient well-being.