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Single-Stranded DNA Synthesis Service 
CRISPR/Cas9 technology is rapidly evolving in an unprecedented way in the field of gene editing, with its core components: gRNA and Cas9 endonuclease. Cas9 targets the specific sequence in the genome under gRNA guidance and cleaves the DNA duplex at the target site to form a DSB nick. This, in turn, triggers DNA repair mechanisms: non-homologous end-linking (NHEJ) or homologous directed repair (HDR). The NHEJ pathway introduces mutations at the DSB site, and in the presence of homologous sequences, the HDR pathway introduces gene knockin at the DSB site.
Studies have shown that when creating a CRISPR knockin, single-stranded DNA (ssDNA) as an HDR repair template has higher specificity and editing efficiency than dsDNA. CRISPR/Cas9 PlatformCB provides fast and affordable ssDNA synthesis services to a wide range of research workers. By performing pure enzymatic synthesis using asymmetric polymerase chain reaction (aPCR), we are able to provide ssDNA greater than 10 kb. As a leading biotechnology company in the field of genetic editing, we guarantee the high quality and purity of our product lines.
DSB breaks created by CRISPR/Cas9 and HDR
The benefits of using ssDNA as a template compared to dsDNA
- Significantly reduce the possibility of random integration into the genome, thereby increasing the efficiency of gene editing
- Low cytotoxicity
- Easily identify correctly edited clones for improved editing accuracy
- Reduce off-target integration
- Ideal for editing primary and stem cells
- Ideal for developing transgenic animal models
Applications of ssDNA
- CRISPR-mediated gene knockin
- In vitro transcription
- In vitro mutagenesis
- Nuclease S1 localization
- Probe preparation and labeling
- Subtractive hybridization and other molecular techniques
Why choose CRISPR/Cas9 PlatformCB?
- Sequence verification of the final ssDNA product by next generation sequencing
- No need to use irritating chemicals, enzymatic methods ensure the detection of dsDNA levels and minimize DNA base damage
- Provide purification to ensure the highest purity
- Free lifetime gene template storage for faster, more cost-effective reordering
As a leading biotechnology company, CRISPR/Cas9 PlatformCB is committed to providing a comprehensive range of CRISPR/Cas9 products and services to support the development of basic research, biotechnology engineering, pharmaceutical research, and basic medical research. You can be confident that we are able to provide sequence-verified ssDNA fragments for CRISPR-mediated gene knockin, in vitro transcription and more. We guarantee the most reliable and efficient research services to best meet your research goals, reduce turnaround time and lower prices. If you have any questions, please feel free to contact us.