Custom Drosophila Model    

CRISPR/Cas9 system has proven to be an efficient genome editing tool for the study of biological problems of diverse organisms and cell types. Drosophila is an advanced medical tool, one of the best model organisms favored by biomedical researchers, for studying many biological problems related to development, genetics, cell biology, neuroscience, and disease.

CRISPR/Cas9 Platform^CB, a global leading biotechnology company in the field of genetic editing, provides customized CRISPR gene editing services for researchers working with Drosophila. We provide efficient means to identify gRNA targets in the sequences you provide and delivery gRNA and Cas9 into Drosophila embryos for target gene editing at the target locus. Each project will be designed, executed and supervised by Ph.D. scientists to ensure the quality and reliability of the entire experimental process.

Workflow of custom Drosophila model

CRISPR/Cas9 Platform^CB offers a CRISPR Drosophila service package that includes design, cloning, microinjection, screening and population building to bring you genetically edited Drosophila. Just let us know the name of the gene and how you want to edit it. We propose the best strategy and complete the rest of the steps for you.

• Consult our genome engineering design experts to establish an exact custom CRISPR/Cas9 gene editing strategy.
• Select guide RNA target sites and synthesize gRNA.
  ➢ gRNA efficiency is tested in S2 or Kc cell lines before making the gene editing Drosophila you want.
• Design and synthesize donor DNA (if needed).
• CRISPR vector construction.
• Plasmid microinjection by our skilled injection specialist (always >150 embryos to fulfill the guarantee).
• Screening and identification (via PCR and sequencing).
• Deliver the final Drosophila model and validation report, which describes the details of the genome editing and shows evidence of the required editing.

Our customized CRISPR/Cas9 Drosophila model service include

• Mutation via CRISPR Gene Editing

CRISPR/Cas9 Platform^CB is able to perform specific DNA deletions, mutations or substitutions to study gene function with CRISPR/Cas9 gene editing technology with high precision. Available services:

➢ Knockout
➢ Point Mutation
➢ Conditional knockout/knockin
➢ Floxed Allele insertion

• Mark by CRISPR gene editing

Using the CRISPR/Cas9 gene editing technology, CRISPR/Cas9 Platform^CB is able to tag your genes at the endogenous locus and visualize them with fluorescent proteins or immunotags for biochemical studies. Available services:

➢ Fluorescent tag (e.g. GFP, mCherry, RFP, etc.)
➢ Immunotag (e.g. Flag-tag, HA-tag, etc.)
➢ Custom tags and combinations are also available

Applications of CRISPR/Cas9 Drosophila

• Functional genetic research

➢ Used as a model to study several mechanisms fundamental to cancer development
➢ Used to model many neurodegenerative diseases such as Huntington's, Alzheimer's and Parkinson's diseases. Construct neural, cardiac and renal disease models to study the molecular mechanisms of metabolic diseases

CRISPR/Cas9 Platform^CB is a specialist provider of gene editing solutions for in vivo and in vitro disease model construction, target gene knockout/knockin/point mutation. With years of expertise, our scientists provide clients with many high-quality and powerful animal and cell models to study disease development mechanisms, gene function, drug screening, and treatment strategy. There is no doubt that CRISPR/Cas9 Platform^CB is the best partner during your project.