Cas Enzyme Production Service    

Background

Cas proteins (CRISPR-associated proteins) are DNA or RNA-binding proteins near CRISPR sequences that also have nuclease functions. These proteins enable programmable genome editing and epigenetic regulation, with applications in homologous-directed repair, gene knockout, chromosomal translocation, single-base editing, chromatin remodeling, and clinical research.

Despite the powerful capabilities of Cas proteins in gene editing, there are limitations. Different Cas proteins vary in cleavage efficiency and specificity, affecting editing accuracy and efficiency. The delivery efficiency of the CRISPR-Cas system in vivo is relatively low, necessitating the development of more efficient vector systems. Off-target effects and immune response issues also need to be addressed to ensure safety and efficacy in clinical treatments. In this regard, high-quality, high-purity Cas enzyme production and customization services become essential.

Service Content

Creative Biogene's Cas Enzyme Production Service

Creative Biogene's Cas enzyme production service offers a range of Cas proteins, guaranteeing clients receive effective and precise gene editing instruments catered to their particular experimental requirements. We provide all-encompassing support, ranging from R&D to GMP production, guaranteeing goods fulfill the caliber requirements for diverse gene editing applications. Let's learn more about it!

Do you have similar needs?

➢ About Customization

Customer: "We need specific types and specifications of Cas enzymes for our unique gene editing needs."

Creative Biogene: "We offer the production of various Cas enzymes, including the widely studied categories like Cas9, Cas12, and Cas13, along with their subtypes. We also support iterative enzyme development, including precise site-directed mutations, knockouts, insertions, and point mutations. This helps in the development and production of new Cas enzymes, reducing operational complexity, and enhancing editing accuracy and success rates."

➢ About Process Transition and GMP Production

Customer: "As we progress from research and development to process development and clinical production, we require smooth process transition support."

Creative Biogene: "Our GMP materials are primarily based on RUO (Research Use Only) products. Our custom service team can convert RUO materials into GMP-compliant products, ensuring product quality and compliance. We continuously expand our gene editing product range, lowering the technical barriers of gene editing to meet various experimental needs and regulatory requirements, making gene editing more accessible."

➢ About Technological Innovation and Market Competitiveness

Customer: "We want to enhance our competitiveness in the research and clinical markets through innovative technological solutions and high-quality Cas enzyme products."

Creative Biogene: "Our services support you with reliable and consistent technologies, helping you achieve market-leading positions."

➢ About Optimized RNP Complex Delivery

Customer: "We need to minimize off-target effects during gene editing to ensure editing precision and safety. This is particularly important in our gene editing research, clinical trials, and commercial production."

Creative Biogene: "We select the appropriate Cas proteins and sgRNA sequences based on your needs and assemble stable RNP complexes. By optimizing delivery methods and conditions, we improve transfection efficiency and editing success rates while reducing off-target effects. After laboratory validation and optimization, we scale this method up to production levels, ensuring stable editing quality and reliability."

Feature

Creative Biogene's Confidence Comes from Strict Quality Assurance

We utilize advanced production and quality control technologies to ensure the quality and stability of each batch of Cas enzyme products. All products are manufactured and QC tested in GMP-compliant facilities and rigorously verified to meet high standards.

• Purity: >95%, determined by SDS-PAGE and SECHPLC.
• Host Protein Residue: ≤10 ng/mg protein, tested by ELISA.
• Host Nucleic Acid Residue: ≤1 ng/mg protein, tested by qPCR.
• Sterility: Verified by membrane filtration methods, meeting sterility requirements.
• Endotoxin: Less than 10 EU/mg, tested by LAL assay.
• Betalactam Free: Ensured to contain no betalactam substances.
• Batch Consistency: Guaranteed through stringent quality control tests.
• Animal Origin Free: No animal-derived peptone or lactose is used in production.

Advantages of Our Service

• Fermentation with various specifications
• Mature GMP regulatory system
• Rich experience in protein expression
• Strict control to achieve batch consistency
• Strictly prevent exogenous contamination
• One-stop customized technical service

Process

Cas Enzyme Production Development Process

1. Requirement Analysis and Definition

We begin with in-depth discussions to understand your specific needs for Cas enzymes, including the type (e.g., Cas9, Cas12, Cas13), purity levels, application scenarios, and desired production scale.

2. Cas Enzyme Gene Design and Optimization

Based on your requirements, we design and optimize the Cas enzyme gene sequences. We then select the most suitable host systems (e.g., E. coli, mammalian cells) for effective expression and production.

3. Cas Enzyme Expression and Purification

The Cas enzyme genes are expressed in the chosen host system. We optimize the expression conditions and then purify the Cas enzymes to achieve high purity using techniques such as centrifugation, column chromatography, affinity chromatography, and size exclusion chromatography.

4. Activity and Stability Testing

We test the activity and stability of the purified Cas enzymes to ensure they provide high-efficiency genome editing and long-term stability. This includes evaluating genome editing efficiency, assessing off-target effects, and verifying stability under various temperature and storage conditions.

5. Quality Control and Certification

The Cas enzyme products undergo stringent quality control processes, including SDS-PAGE and SECHPLC analysis for purity and composition. We also perform endotoxin testing (LAL assay) and measure host protein and DNA residues to ensure the products meet industry standards.

6. Scaling Up to GMP Level Production

After validating and optimizing the production process at the laboratory scale, we scale up to GMP-compliant production. This involves making further process adjustments and thorough documentation to ensure product quality, stability, and consistency in large-scale production.

7. Technical Support and Customer Service

We offer continuous technical support and customer service, providing advice on process optimization, application consulting, and problem-solving to ensure you achieve the best results with our Cas enzyme products.

Figure 1. Creative Biogene's Cas enzyme production process.

Contact Us

Whether you're engaged in basic research or pursuing clinical applications, Creative Biogene's Cas enzyme production services offer unparalleled support. Our expertise ensures that you receive high-quality, customized Cas proteins tailored to your specific needs, enhancing the precision and efficiency of your gene editing projects. With our comprehensive range of services—from initial R&D to full-scale GMP production—and our commitment to rigorous quality assurance, we stand ready to support your scientific endeavors. Contact us today to discover how we can elevate your research and clinical outcomes!