Custom C. elegans Model    

CRISPR/Cas9 has been successfully applied to Caenorhabditis elegans and allows rapid mutant recovery, creation of endogenous locus tagged versions, and implementation of conditional knockout and knockin. Although certain restrictions on target DNA may be beyond the requirements of PAM, CRISPR/Cas9 allows almost any gene to be deleted and is edited with precision and fidelity.

CRISPR/Cas9 Platform^CB has maintained close cooperation with C. elegans researchers. In order to promote the progress of related projects, a customized CRISPR/Cas9 gene editing C. elegans model is launched. We design, build and deliver genetically engineered C. elegans that can be optimized to your specifications. We also collaborate on project design to ensure transparency in the genome editing process and to ensure that you receive the transgenic that is best for your experiment.

Our customized CRISPR/Cas9 C. elegans model service include

• Mutation via CRISPR Gene Editing

CRISPR/Cas9 Platform^CB is able to perform specific DNA deletions, mutations or substitutions to study gene function with CRISPR/Cas9 gene editing technology with high precision. Available services:

➢ Knockout
➢ Point Mutation
➢ Conditional knockout/knockin
➢ Floxed allele insertion

• Mark by CRISPR gene editing

Using the CRISPR/Cas9 gene editing technology, CRISPR/Cas9 Platform^CB is able to tag your genes at the endogenous locus and visualize them with fluorescent proteins or immunotags for biochemical studies. Available services:

➢ Fluorescent tag
➢ Immunotag
➢ Custom tags and combinations are also available

Workflow of custom C. elegans model

• Consult our genome engineering design experts to establish an exact custom CRISPR/Cas9 gene editing strategy.
• Select guide RNA target sites and synthesize gRNA.
• Design and synthesize donor DNA (if needed).
• CRISPR vector construction.
• Plasmid microinjection by our skilled injection specialist (typically we inject as many embryos as needed to fulfill the guarantee).
• Screening and identification (via PCR and sequencing).
• Deliver the final C. elegans model and validation report, which describes the details of the genome editing and shows evidence of the required editing.

Applications of CRISPR/Cas9 C.elegans

• Study the neurodevelopment of animals
• Construct neural, cardiac and renal disease models to study the molecular mechanisms of metabolic diseases
• High-throughput drug screening

Our advantages

• High-efficiency gRNA for ~99% Cas9 target cutting efficiency
• Efficient CRISPR strategy and protocol for gene editing
• F1 breeding to ensure germline transmission
• Timely report on project progress
• Convenient and efficient transportation service
• Shorter turnaround time and lower price

Why use a C. elegans?

• Very small and the life cycle is short.
• It is transparent throughout its life and can be used to track the behavior of alternating cells.
• Many of the molecular signals that control its development also exist in more complex organisms such as humans.
• Many of the genes in the C. elegans genome have functional counterparts in humans, making them extremely useful models of human disease, including neurological diseases, congenital heart disease, kidney disease, and high blood pressure.

As one of the leading experts in the field of genetic engineering, CRISPR/Cas9 Platform^CB is very professional in providing CRISPR/Cas9 technology application solutions. Based on the microinjection and gene editing technology platform and an excellent team of experts, we are committed to providing our customers with the most reliable and efficient research services to best match their research goals. Want to know more? Pleases feel free to contact us.