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CRISPR/Cas Application in Bacterial Industrial Evolution 
Understanding bacterial genetic mechanisms through genome editing would enable researchers to better explore the physiological and metabolic underpinnings of many organisms. Especially important for bacteria of scientific and economic importance, conventional approaches, like mutating a single amino acid, are often imprecise. But with the development of CRISPR-Cas systems, bacterial genome editing has undergone a revolutionary change that offers previously unheard-of precision and versatility.
CRISPR-Cas Systems' Effect on Bacterial Genomes
In bacterial genome editing, CRISPR-Cas systems offer a paradigm change that allows for remarkably efficient and accurate modification. This development offers up new avenues for biotechnological and industrial applications while also promising quick advancements in the study of bacterial genomes. Thus, the finding of bacterial genome editing has enormous implications for industrial production and biotechnology in the future, which will change the direction of scientific inquiry and its uses.
Figure 1. CRISPR-Based Technologies for Bacterial Genome Editing (Krink N, et al., 2024)
Advancements in CRISPR for Industrial Microbial Engineering
CRISPR-based gene editing is revolutionizing industrial microbial engineering by offering targeted genetic enhancements to chassis microorganisms like Escherichia coli, Corynebacterium glutamicum, Bacillus subtilis, and Saccharomyces cerevisiae. Since the validation of the CRISPR/Cas9 system targeting the E. coli genome in 2013, these technologies have been extensively employed to boost productivity. For example, Li et al. achieved nearly 100% editing efficiency in E. coli, optimizing pathways for β-carotene production and obtaining a yield of 2.0 g/L. Furthermore, CRISPR has facilitated the engineering of probiotic strains like E. coli Nissle 1917 for therapeutic applications, paving the way for efficient live therapy design. These advancements highlight CRISPR's pivotal role in enhancing industrial microbial performance, promising further innovations in the field.
Figure 2. Example workflow for CRISPR-mediated bacterial genome editing for industrial production. (Kolasinliler et al., 2023)
Bacterial genome editing with CRISPR involves several crucial steps for successful genetic modification and industrial production. Firstly, target prediction identifies relevant genes in metabolic pathways. These genes are then silenced or induced. Next, the CRISPR construct is designed by selecting Cas proteins, designing gRNAs, and choosing suitable promoters and terminators. Constructs can vary in format, such as single or multiple plasmids containing Cas protein, gRNA, and donor DNA fragments. Constructed CRISPR plasmids are delivered into bacterial cells via transformation methods, with electroporation preferred for its high efficiency. Genome editing can be achieved through various methods like CRISPR-Cas, CRISPRi, CRISPRa, base editing, or prime editing, depending on the chosen strategy. Finally, clones with desired plasmids are selected using techniques such as antibiotic resistance screening or fluorescence microscopy.
Microbial Genome Editing Services by Creative Biogene's CRISPR PlatformCB
Comprehensive Microbial Genome Editing Services
Explore the wide world of genetic engineering with our all-inclusive services for editing the microbial genome. Our CRISPR-mediated method works with a broad variety of microorganisms, such as filamentous fungi, Salmonella, Corynebacterium glutamicum, Clostridium, Bacillus subtilis, and Escherichia coli.
Streamlined Operation for Your Convenience
Our intuitive interface makes editing easier. Just tell us the strain you wish to change, any pertinent details, and the target gene names or sequences.
Advantages of CRISPR PlatformCB
- Multi-Gene Editing: We can knock out multiple genes at once, allowing for more intricate genome modifications and providing our clients with a variety of options.
- Simple Selection: Our services don't require antibiotic-resistant target bacteria, which lowers potential safety risks and makes it easier for clients to conduct follow-up experimental procedures.
- Tailored Services: We customize genome editing options to meet the unique needs of each client. This includes creating species-specific CRISPR genome editing systems or applying standard homologous recombination techniques.
- Time and Cost Efficiency: With our state-of-the-art laboratory apparatus and extensive technical support, we can provide our clients with high-quality services at a reasonable cost promptly, saving them both money and time.
Related Services and Products
E. coli Genome Editing Service
Microbial Genome Editing Services
Base Editing by CRISPR
SgRNA Design and Confirmation
From Basic Research to Industrial Application
Our services are remarkably impactful and versatile, bridging the gap between industrial application and research with ease.
- Production of Industrial Enzymes and Antibiotics: By using genome editing, clients can modify microbial strains to produce specific enzymes and antibiotics, meeting the demands of the pharmaceutical and industrial sectors.
- Microbial Ecological Regulators for Food Fermentation: Through the modification of microbial strains, fermentation processes are optimized, improving quality and performance for the production of food products while guaranteeing dependability.