CRISPR Rat for Immunology Research    

The rat is an important animal model for understanding gene function and developing human disease models. The larger body size of rats provides greater blood volume and facilitates surgical implantation compared to mice. In addition, immunological characteristics are more similar between rats and humans than between mice and humans. For these reasons, the development of humanized immunodeficient rat models is valuable and widely used in tumor immunology research.

To date, rapid advances in genome engineering technologies have led to the creation of many animal models of immunodeficiency with a wide range of applications. the CRISPR/Cas9 system has been successfully applied to genetic modification of endogenous target genes (e.g., gene-specific knockout and knock-in) in a variety of organisms, including the rat. the CRISPR/Cas9 system, due to its simple application and ease of use in genetic modification, is now widely used worldwide. CRISPR rats have been widely used worldwide as attractive experimental animals for immunological and pharmacological research.

Fig. 1 Generation of Il2rg and Rag2 knockout rats using CRISPR/Cas9. (Miyasaka Y, et al., 2022)

Solution

CRISPR/Cas9 Platform^CB creates high-quality immunodeficient rats that researchers can freely use. Our global portfolio of high-quality immunodeficient rat models can advance your research in tumor biology, immunology, and xenografts. To help you determine the most appropriate immunodeficient rat model for your oncology studies, we offer animal models with different immunodeficiency levels and phenotypic characteristics, including but not limited to B-Rag2 KO rats, B-Rag2/IL2rg KO rats, B-IL2rg KO rats, RNU Nude rats, SRG rats, double knockout F344-scid gamma (FSG) rats.

Benefits of Our CRISPR Rat for Preclinical Tumor Immunology Research

• High implantation rates and rapid growth of multiple human tissues and tumors.
• Severe immunodeficiency ensures high implantation rates and is suitable for reconstruction with human PBMC.
• Larger organism size allows for greater tumor load and reduces the difficulty of procedures such as cannulation and blood collection.
• Larger tumor size allows for continuous tissue sampling from the same animal throughout the treatment.

Advantages of Our Technology

• Higher integration efficiency
• Higher probability of target gene expression
• Greater insertion of target fragments
• More accurate copy number insertion
• Free removal of inserts

Related Services

• Knockout Rat Model Service
• Knock-in Rats Model
• Point Mutation Rat Model Service

Related Products

• CRISPR/Cas9 Kits
• Cas9 Protein
• Cas9 mRNA
• Cas9 Related Plasmids
• Pre-made Cas9 Virus Particles

CRISPR/Cas9 Platform^CB provides a one-stop solution for all your gene-edited rat modeling needs. Our immunodeficient rats serve as an effective preclinical model for studying lymphatic compartment development, B-cell malignancies (e.g., leukemia, lymphoma), and the kinetics and molecular mechanisms of cancer transmission. You can contact us to perform your entire gene-edited project, from initial strategy design and DNA vector construction to gene-edited animal delivery, or you can outsource specific phases of your project to us. We are looking forward to working with you.