Point Mutation Rat Model Service    

CRISPR/Cas9 Platform^CB is a leading biotechnology company focused on gene editing. We can provide solutions for in vivo and in vitro target gene modification and CRISPR system applications, including comprehensive processes such as screening of optimal target sites, guide RNA design and synthesis, vector construction, and quality control.

CRISPR/Cas9 Platform^CB provides you with a CRISPR/Cas9 point mutation rat model construction service. Our team of experienced experts can help you understand what you are investigating and provide you with the most suitable solution.

Strategy of CRISPR/Cas9 point mutation rats

Inject gRNA, Cas9 mRNA and ssDNA into rat fertilized eggs at the same time. ssDNA acts as a DNA repair template, introducing point mutations into target sites during homology-directed repair (HDR).

The workflow of point mutation rat model

• Step 1. Strategy design

Tell us your gene name and point mutations required for knock-in. Our scientists will select the most suitable target sites, maximize the nuclease activity on the target, and minimize off-target reactivity to design the nucleic acid.

• Step 2. sgRNA, ssDNA design and synthesis

Our scientists will optimize your sgRNA sequence to maximize efficiency and minimize off-target effects, construct the nuclease expression vector, and design donor templates and primers for PCR and sequencing-based genotyping analysis.

• Step 3. Microinjection

CRISPR vectors injection to get point mutation knock-in founder rats.

• Step 4. Validation

➢ Genotype puppies to identify the founder of knockout genes by PCR and sequencing.
➢ Off-target analysis
➢ Breed the founders to get F1 (optional)

• Step 5. Delivery

Delivery the correctly edited rats and final report containing all details about experiment program.

The applications of point mutation rats

For academic research	For biopharmaceuticals research and development
Study the functions of specific mutant genes/proteins	Human disease models (including genetic diseases, neurological diseases, cancer, etc.)
Analysis of the role of non-coding regions and regulatory factors	Study drug-resistant mutants
Screening and verification of disease-causing mutations	Study drug-antibody affinity
Study resistant mutations	Pharmacology off-target and efficacy studies,
	Preclinical safety and toxicity studies

Main features

• Latest CRISPR design strategy
• 100% target cleavage efficiency with proven gRNA
• Very successful in generating the final rat model
• F1 breeding to confirm germline transmission

CRISPR/Cas9 Platform^CB is a pioneer in the frontier of human disease model building. With the help of an experienced team of experts and a professional technology platform, using the CRISPR/Cas9 system, we provide customers with high-quality point mutation rat construction services. CRISPR/Cas9 Platform^CB provides our customers with the most effective and reliable services to best meet their needs and promote the development of the project. If you have any questions or requirements, don't hesitate to contact us.