Drug Screening in Gene-Edited Cells    

Overview

Cell models play a crucial role in drug screening. Through rapid and precise efficacy assessment and simulation of complex disease environments, they effectively identify potential drug candidates. These models not only aid in understanding drug mechanisms and effects but also support personalized medicine and precision drug development. This advances translational medical research and provides new scientific foundations and treatment strategies for various diseases.

Challenges in Current Cellular Model Screening Methods

The limited availability of cell line resources, especially for simulating specific genetic mutations or disease types, poses a significant challenge in selecting appropriate cell models. Additionally, existing screening methods often lack high-throughput and high-content evaluation capabilities, leading to time-consuming processes, high costs, and difficulties in comprehensively assessing complex cellular signaling pathways and disease phenotypes.

Creative Biogene Addresses These Challenges

Creative Biogene offers solutions that include using advanced gene-editing technologies such as CRISPR/Cas9 to design and construct cell lines with specific genetic mutations. These customized cell models accurately replicate complex genetic disease scenarios and support personalized medicine and precision drug development. We provide an extensive range of cell line resources (46,000+ cell lines) covering various disease models and ensure the accuracy and reliability of experimental data through stringent quality control and validation processes.

Figure 1. Early-stage drug discovery approaches. (A) and (B) illustrate two primary approaches in drug discovery: target-based drug discovery (TDD) and phenotypic drug discovery (PDD). (Tang Q, et al., 2024)

Service Content

Gene-Edited Cell Model Drug Screening Service Overview

Creative Biogene offers Gene-Edited Cell Model Drug Screening Services, utilizing advanced gene-editing technology and cellular phenotypic analysis methods to help clients efficiently evaluate drug efficacy and safety in specific genetic mutation scenarios. Our services include target-based drug discovery (TDD) and the latest advances in phenotypic drug discovery (PDD). By combining the strengths of TDD and PDD, we offer diverse drug screening services covering the entire process from gene-edited cell line construction to drug screening and data analysis, addressing the treatment challenges of complex diseases and advancing personalized medicine and precision drug development.

Service Offerings

1. Gene-Edited Cell Line Construction:

We use CRISPR/Cas9 technology to design and construct cell lines with specific genetic mutations based on clients' specific needs and research goals. These gene-edited cell lines cover various disease models, including cancer, immune modulation diseases, and genetic disorders.

• Precise Gene Editing Design: High-precision gene-editing schemes are designed based on client-provided gene information and research objectives.
• Cell Line Validation: Strict experimental procedures and quality control standards ensure the accuracy and stability of each gene-edited cell line.

2. Cellular Phenotypic Screening:

Using various cellular phenotypic analysis methods, we comprehensively evaluate gene-edited cell lines to determine drug efficacy and mechanisms of action.

• Cell Viability Assays: Assess drug activity against cancer cells or exogenous pathogens, accelerating the drug screening process.
• Cell Signaling Pathway Assays: Analyze the impact of drugs on specific signaling pathways, helping clients identify potential therapeutic targets.
• Disease-Relevant Phenotype Assays: Simulate various disease models to explore drug efficacy and safety under complex genetic variations.

3. Advanced Imaging and Data Analysis:

We employ advanced imaging technologies and high-content data analysis methods to provide clients with detailed experimental data and in-depth data interpretation.

• Image-Based Analysis Methods: Use high-content morphological annotation and deep learning technologies to construct gene function networks and understand the relationship between drugs and disease-related phenotypes.
• Live Cell Phenotypic Analysis: Time-series imaging accurately predicts drug mechanisms and reveals dynamic cell state transitions.

4. Customization and Client Support:

We offer flexible experimental design templates and customized service solutions based on clients' specific research needs. Throughout the research process, we work closely with clients to ensure data quality control and the achievement of research goals.

Advantage

Service Advantages

• High Throughput and Cost-Effectiveness: Screen a large number of gene-edited cell lines per run, optimizing research costs and shortening development cycles.
• Comprehensive Service Package: From gene editing to drug screening and data analysis, we provide one-stop solutions covering the entire research process.
• Professional Experimental Support: An experienced team offers expert experimental support and technical guidance, ensuring smooth research progress and data accuracy.

Contact Us

Creative Biogene is committed to providing high-quality experimental tools and solutions for global scientific research through advanced gene-editing technologies and cellular phenotypic analysis methods. Our services not only enhance the efficiency and accuracy of drug screening but also advance scientific progress in the biomedical field, offering crucial support and guidance for new drug development. Choose to collaborate with us and embark on a new chapter of scientific exploration! For more information about our Gene-Edited Cell Model Drug Screening Services, please contact our customer service team.