CRISPRa Cell Line    

CRISPR activation (CRISPRa) is an important tool for interfering with transcription, providing new strategies for exploring gene regulation research. Creative Biogene CRISPR/Cas9 Platform^CB has long been committed to the field of gene editing as a leading provider. We have created high-quality CRISPRa cell line products using state-of-the-art technology. Our CRISPRa cell lines have precise and efficient gene activation capabilities, making them ideal for a wide range of gene function studies. This transformative approach helps our customers make progress in related fields.

Introduction of CRISPRa

CRISPRa, also known as CRISPR activation, works by binding dCas9 to various transcriptional activators to increase the expression of target genes. dCas9 can activate endogenous promoters for efficient transcription without the need for additional exogenous expression elements. It is not limited by gene transcript size. Compared to traditional overexpression techniques, CRISPRa promotes gene expression by activating endogenous promoters for efficient transcription without the need to construct additional exogenous expression elements. Additionally, it is not limited by the size of the gene transcript. dCas9-mediated gene regulation is reversible and does not permanently modify the genomic DNA. Furthermore, it is possible to design multiple gRNAs to regulate multiple target genes simultaneously. Additionally, fusing dCas9 with fluorescent proteins enables tracking of target gene location.

Fig. 1 Schematic diagram of the CRISPRa system. (Kampmann, M., 2018)

Overview of Our CRISPRa Cell Lines

To create CRISPRa cell lines, we combined catalytically inactivated Cas9 (dCas9) proteins with transcriptional activators. This was done to precisely activate the target gene in the host cell line. We then integrated this fusion construct into the genome of the target cell line using a lentiviral transduction approach. To generate a stable CRISPRa cell population with the correct protein expression levels, we used advanced screening techniques to obtain a monoclonal cell population. We then tested the CRISPRa activity using a functional assay. Our high-quality CRISPRa cell lines provide reliable and reproducible results for our clients, enabling researchers to achieve innovative results in the field of gene regulation.

Applications of CRISPRa Cell Lines

• Functional genomics studies
• Disease modeling
• Potential disease target identification
• Pathway analysis
• Drug screening
• Gene therapy research

Why Choose Us?

• Efficient and stable cell lines enable targeted, robust activation of specific genes.
• CRISPRa cell line products derived from a wide range of host cell lines can fulfill a wide range of research demands of different customers.
• Strict quality control ensures efficiency, specificity and stability of gene activation.

Creative Biogene CRISPR/Cas9 Platform^CB is committed to providing our customers with the CRISPRa cell line products they require. By selecting our CRISPRa cell lines, you gain access to potent gene function research tools to advance your innovative research and make progress in your field. Please contact us for more information, including product availability, pricing and customization options.