Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
CHO Cell Antibody Drug Production Service 
Background
Therapeutic antibodies are highly regarded for their high affinity, specificity, low immunogenicity, and ability to target a wide range of biological molecules. Currently, mammalian cells, including Sp2/0, NS0, HEK293, and CHO cells, are primarily used for the production of therapeutic antibodies, with CHO cells accounting for over 70% of the production due to their complex posttranslational modification capabilities.
CHO cells are widely chosen for several reasons:
- Similar posttranslational modifications to human cells.
- High-density growth in serum-free and chemically defined suspension culture media.
- Efficient extracellular secretion of products, simplifying downstream purification processes.
- Minimal secretion of endogenous proteins, reducing purification complexity.
- High-level protein expression during extended fermentation periods.
Recombinant antibody production is a key technology for the commercialization of monoclonal antibody drugs, enabling the stable production of large quantities of high-quality antibodies using antibody gene sequences.
Figure 1. The development process of recombinant antibody production using CHO cells. (Majumdar S, et al., 2024)
Antibody Production
CHO Cell Antibody Production Service Content
Creative Biogene's high-throughput recombinant antibody expression and purification services in CHO cells combine high-throughput gene synthesis, vector construction, and optimized transient antibody expression technology. We can rapidly produce recombinant antibodies from milligram to kilogram scale, meeting our clients' high-throughput and large-scale production needs.
High-Throughput Small-Scale Antibody Production Services (0.1mg-1mg)
Creative Biogene offers codon optimization, high-efficiency expression vectors, optimized media, and expression systems, with flexible product quantities and short turnaround times for high-throughput production. Our services can produce over 100 antibody samples per week.
| Service Process (2-4 weeks) |
| 1. Gene synthesis and codon optimization (optional). |
| 2. Transient transfection of CHO cells. |
| 3. One-step affinity purification of antibodies. |
| 4. QC analysis including SDS-PAGE and UV analysis. |
| 5. Delivery of SDS-PAGE report and Certificate of Analysis (CoA). |
Small to Medium-Scale Recombinant Antibody Production Services (1mg-100mg)
Creative Biogene has developed high-efficiency media and transfection reagents, proprietary high-expression vectors, and engineered cell lines to ensure cost-effective and high success rate antibody production. We offer transient transfection for antibody production and the development of stable antibody-expressing cell lines to meet diverse client needs. We can express various isotypes of full-length antibodies, chimeric antibodies, and antibody fragments.
| Service Process (6-8 weeks) |
| 1. The client provides antibody gene sequences or vectors. |
| 2. Gene synthesis and codon optimization (optional). |
| 3. Clone antibody heavy and light chains into optimized expression vectors. |
| 4. Sequencing validation and large-scale plasmid production. |
| 5. Transient transfection of CHO cells. |
| 7. Antibody purification and fine purification. |
| 8. QC analysis including SDS-PAGE and UV analysis. |
| 9. Delivery of SDS-PAGE report and Certificate of Analysis (CoA). |
Large-Scale Recombinant Antibody Production Services (100mg-100g)
Creative Biogene's services ensure cost-effective and high success rate antibody production. We offer continuous 7-day, 24-hour cultivation from shake flasks to bioreactors of various scales, ensuring rapid antibody production. We provide transient transfection for antibody production and the development of stable antibody-expressing cell lines to meet diverse client needs. Customized high-purity, high-concentration, low-endotoxin antibodies are available according to client specifications. We also offer one-stop services from monoclonal antibody preparation to GMP production.
| Service Process (9-12 weeks) |
| 1. The client provides antibody gene sequences or vectors. |
| 2. Gene synthesis and codon optimization (optional). |
| 3. Clone antibody heavy and light chains into optimized expression vectors, perform sequencing validation and produce large quantities of plasmids. |
| 4. Transiently transfect CHO cells and purify antibodies. |
| 5. Perform fine purification and QC analysis as needed. |
| 6. Deliver SDS-PAGE reports and Certificates of Analysis (CoA). |
Advantage
Advantages of Our CHO Cell Antibody Drug Production Service
- Professional Technology and Equipment: Over 50 shakers and 100 bioreactors (2L~1000L) with an annual cell culture capacity exceeding 50,000L.
- Efficient Platform: Free codon optimization, high-efficiency expression vectors, optimized proprietary media, and mammalian cell expression platform.
- Extensive Experience: Multiple expression capabilities, including scFv, Fab, full-length antibodies, chimeric antibodies, bispecific antibodies, and co-expression of multiple chains, covering a wide range of isotype expression and purification experiences.
- Quality Assurance: Monomer purity >99%, endotoxin levels <0.01EU/mg, comprehensive QC analysis capabilities including UV, SDS-PAGE, ELISA, qPCR, SECHPLC, CEXHPLC, LCMS/MS, CESDS, ADCC, CDC, OCTET Affinity, Kinetics, off-rate, and ranking.
Figure 2. Creative Biogene's laboratory
Contact Us
Creative Biogene is dedicated to providing fast, efficient, and reliable CHO cell antibody drug production services. With our professional technology and extensive experience, we can meet all your needs from research to GMP production. Choose Creative Biogene to support breakthrough advancements in your antibody drug development.