Knockout Rat Model Service    

CRISPR/Cas9 Platform^CB provides you with custom rat gene editing services through the CRISPR/Cas9 system and in vitro microinjection platform. We guarantee that at least 2 founders or 3 F1 animals will be eliminated.

What we offered?

• Constitutive knockout rat
• Conditional knockout rat (Loxp labeled)

The workflow of CRISPR/Cas9 knockout rat model

• Step 1. Strategy design

➢ Select most suitable guide RNA target sites
➢ Guide RNA design
➢ Design donor DNA containing Loxp sequences for conditional knockout rats

• Step 2. Synthesis & cloning

➢ Synthesize gRNA
➢ Synthesize donor DNA
➢ Construct CRISPR vector

• Step 3. Microinjection

➢ gRAN designed for a specific site of the target gene and Cas9 are simultaneously injected into the mouse embryos.
➢ Typically, we will inject as many embryos as needed to fulfill the guarantee.

• Step 4. Validation

➢ Raise the young rats
➢ Identification and selection by PCR and sequencing

• Step 5. Delivery

➢ Delivery the correctly edited rats and final report containing all details about experiment program

Our benefits

• Two knockout strategies - Constitutive knockout and tissue-specific/inducible knockout
• More than 3 types of gRNA, optimized gRNA design strategy, maximize efficiency and minimize off-target effects
• F1 breeding to ensure germline transmission
• Timely report on project progress
• Faster turnaround and more economical
• Convenient and efficient transportation service

Applications of CRISPR/Cas9 rat model

• Study the biological functions of genes and proteins
• Building a specific human disease model
• Drug screening and development
• Preclinical safety and toxicity studies
• Verify drug targets and specificities
• Genomic verification of antibodies

CRISPR/Cas9 Platform^CB has continuously innovative research design, best facilities, and strong quality supervision channels, all of which will help our customers to obtain high-quality results at a lower cost and less time. Optimally designed sgRNA can efficiently target the gene-specific PAM site of your interest and minimize off-target effects. Contact us for further support on CRISPR/Cas9 solutions for in vivo and in vitro applications. There is no doubt CRISPR/Cas9 Platform^CB is the best partner during your program.