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CRISPR Rat for Pharmacokinetic Research 
Rats are ideal for many laboratories because they are physiologically similar to humans. Rat models are even more important in pharmacological studies, especially pharmacokinetic (DMPK) studies. The large size of the rat allows for the study of the distance effects of drug administration on specific anatomical regions and allows for continuous blood sampling for drug analysis. In addition, rats have a more similar biology to humans in certain diseases (e.g., diabetes and breast cancer) compared to mice. Therefore, rat models may be the model of choice for drug metabolism in some cases.
Advances in gene targeting and gene editing have allowed modification of the rat genome to produce knockout and knock-in rat models. In recent years, CRISPR-Cas9 has been used to create a series of drug metabolism and DMPK rat models. The novel rat models are not only widely used for drug metabolism, chemical toxicity, and carcinogenicity studies, but also facilitate the study of DMPK-related mechanisms and further strengthen the link between drug metabolism and pharmacology/toxicology.
Fig. 1 Flowchart of gene editing animal model construction based on CRISPR-Cas9 technology. (Lu J, et al., 2021)
Solution
CRISPR/Cas9 PlatformCB is dedicated to enhancing your understanding of pharmacokinetics through the development of robust custom rat models. Our rat models can help you reduce drug failure rates in clinical trials, which can lower development costs and shorten development time. A sequenced genome will also reveal new targets for drug interventions.
Our CRISPR-Cas9 technology allows us to generate multiple rat models including but not limited to Cyp3a1/2 double knockout (KO) rats, Cyp1a2 knockout rats, Ces2a knockout rats, Abcb1 knockout rats, Slco1b2 knockout rats, Oatp1b2 knockout rats, Slc22a6/Slc22a8 double knockout (KO) rat models. Subsequently, we can characterize their viability and physiological status. We can also customize genetic KO rat models to your specifications, and can construct humanized rat animal models for studying the role of certain genes in metabolic diseases, drug metabolism, pharmacokinetics, drug toxicity and carcinogenicity.
Our One-Stop Solution Offerings
We have established a wide range of solutions in the field of gene-edited rats to address pharmacokinetic issues in their applications. With our experienced executive team and dedicated researchers, we can provide you with a one-stop solution.
- Development of knockout rats
- Physiological phenotypic assays, including serum renal function, liver function, lipids, glucose indicators and urinary early kidney injury markers
- Uremic toxin concentration and renal uptake rate assays
- Detection of compensatory effects
- Evaluation of gene function and pharmacokinetic assays after drug application
What Studies Our Models Can Be Used for?
- For drug metabolism and DMPK studies
- For disease studies
- For drug metabolism enzyme studies
- For drug transporter studies
- For drug-drug interaction (DDI) studies
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Reference:
- Lu J, et al. CRISPR-Cas9: A method for establishing rat models of drug metabolism and pharmacokinetics. Acta Pharm Sin B. 2021, 11(10):2973-2982.