Cas9 Stable Cell Line - K562
Cat.No. : CSC-RO0178 Host Cell: K562
Size: >1x10^6 cells/vial Validation: T7 Endonuclease I assay
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Cell Line Information
Safety and Packaging
| Cat. No. | CSC-RO0178 |
| Product Type | Cas9 overexpression stable cell line |
| Introduction | Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR). |
| Cell Line Information | K562-Cas9 cell line is engineered to stably overexpress Cas9 nuclease. The Cas9 nuclease in K562-Cas9 cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, K562-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc. It is also an ideal cell line model for sgRNA screening and validation, either individually or in pools. |
| Target Gene | Cas9 |
| Host Cell | K562 |
| Applications | 1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation |
| Quality Control | 1) T7E1 assay 2) Mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6 cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Case Study
Hemoglobinopathies are a set of hereditary genetic illnesses caused by mutations in the hemoglobin beta (HBB) gene. The researchers used a combined supramolecular nanoparticle (SMNP)/supramolecular nano substrate-mediated delivery (SNSMD) approach to enable CRISPR-Cas9 knockin of the hemoglobin beta (HBB) gene into the adeno-associated virus integration site 1 (AAVS1) safe-harbor site of an engineered K562 3.21 cell line carrying the sickle cell disease mutation. This required the successive delivery of SMNP vectors harboring a Cas9•sgRNA combination and an HBB/green fluorescent protein (GFP)-encoding plasmid. Successful CRISPR-Cas9 knockin by HDR was performed, and further in vivo investigations confirmed the proliferative capability of HBB/GFP-knockin K562 3.21 cells after intraperitoneal injection into mice, laying the framework for broad gene therapy applications in hemoglobinopathies.
Figure 1. The researchers employed a combined SMNP/SNSMD approach to deliver EGFP-Cas9•sgRNA or HBB/GFP plasmid into K562 3.21 cells. (Yang P, et al., 2020)
Accelerate your gene editing research! Creative Biogene's Cas9 stable cell line K562 provides you with a stable and efficient platform for CRISPR-Cas9 gene editing experiments. This advanced cell line ensures continuous expression of Cas9, eliminating the need for repeated transfections, simplifying the experimental process, and improving the accuracy and reproducibility of gene editing. Whether it is used for gene modification of disease models or gene therapy research, Cas9 stable cell line-K562 is an indispensable tool for you. Accelerate your research progress, choose Creative Biogene's Cas9 stable cell line K562, and start your innovative gene editing journey!
For research use only. Not intended for any clinical use.
