Cas9 Stable Cell Line - HepG2
Cat.No. : CSC-RO0030 Host Cell: HepG2
Size: >1x10^6 cells/vial Validation: T7 Endonuclease I assay
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Cell Line Information
Safety and Packaging
| Cat. No. | CSC-RO0030 |
| Product Type | Cas9 overexpression stable cell line |
| Introduction | Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR). |
| Cell Line Information | HepG2-Cas9 cell line is engineered to stably overexpress Cas9 nuclease. The Cas9 nuclease in HepG2-Cas9 cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, HepG2-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc. It is also an ideal cell line model for sgRNA screening and validation, either individually or in pools. |
| Target Gene | Cas9 |
| Host Cell | HepG2 |
| Applications | 1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation |
| Quality Control | 1) T7E1 assay 2) Mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6 cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Applications
Cas9 stable cell line-HepG2 has multiple applications in various fields of scientific research, especially in the fields of genomics and molecular biology. From perspective, here are some applications:
Gene editing: The main application of Cas9 Stable Cell Line-HepG2 is CRISPR-CAS9-mediated gene editing. The researchers used the tool to introduce or delete specific DNA sequences in the genome of HepG2 cells. This advanced technology allows scientists to study gene function and genetic variation.
Targeted gene knockout: Cell lines generate in vitro models by knocking out specific target genes. This aids scientific research by helping to understand disease mechanisms and for use in drug development studies.
Functional genomics: The Cas9 stable cell line-HepG2 is also used for functional genomics studies. With the ability to edit any gene, scientists can unlock and evaluate the performance of individual genes in the genome.
Disease modeling: Given that HepG2 is originally derived from human liver cancer cells, this cell line is used for human disease modeling, particularly liver-related diseases. By introducing specific genetic alterations, researchers can create disease models to study pathology and potential treatments.
Drug screening: Cas9 stable cell line-HepG2 is an excellent platform for drug screening and toxicology research. Given their genetic modification capabilities, these cells can be used to study drug efficacy and potential toxicity.
For research use only. Not intended for any clinical use.
