Cas9 Stable Cell Line - THP1
Cat.No. : CSC-RO1123 Host Cell: THP1
Size: >1x10^6 cells/vial Validation: T7 Endonuclease I assay
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Cell Line Information
Safety and Packaging
| Cat. No. | CSC-RO1123 |
| Product Type | Cas9 overexpression stable cell line |
| Introduction | Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR). |
| Cell Line Information | THP1-Cas9 cell line is engineered to stably overexpress Cas9 nuclease. The Cas9 nuclease in THP1-Cas9 cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, THP1-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc. It is also an ideal cell line model for sgRNA screening and validation, either individually or in pools. |
| Target Gene | Cas9 |
| Host Cell | THP1 |
| Applications | 1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation |
| Quality Control | 1) T7E1 assay 2) Mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6 cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
THP1 cells are a spontaneously immortalized monocyte-like cell line derived from the peripheral blood of a 1-year-old patient with monocytic leukemia and are an important model in immunology and cancer research. The THP-1 monocyte cell line is known for its ability to differentiate into mature macrophages and dendritic cells and is critical for studying the function and properties of these immune cells in vitro, including adipose tissue macrophages and M2 mononuclear phagocytes. THP-1 macrophages can polarize into M1 or M2 macrophages, which is critical for the study of immunity and inflammation, innate immunity, and inflammatory responses.
Cas9 Stable Cell Line - THP1 is a genetically engineered cell line that is highly durable and efficient for gene editing applications. The cell line contains the Cas9 protein, a key component of the CRISPR-Cas9 gene editing system, stably integrated into its genome. The Cas9 protein is an RNA-guided DNA endonuclease that enables highly specific gene editing. Due to the stability of Cas9 integration, these cells can perform robust and reliable gene editing operations. The Cas9 stable cell line-THP1 was cultivated to ensure consistent expression of Cas9 protein, which greatly facilitates the CRISPR-Cas9 gene editing process. These cells are valuable tools for researchers aiming to study gene function, create disease models, or develop new treatment strategies.
Cas9 Stable Cell Line - THP1 can be widely used in many fields of genetic and cellular research.
Gene Editing: The primary application of Cas9 Stable Cell Lines - THP1 is gene editing. Researchers can use these cells to insert, delete or replace specific DNA segments in specific genes through the CRISPR-Cas9 system.
Disease Modeling: The Cas9 Stable Cell Line - THP1 can be used to model human diseases, especially those with a genetic basis. It allows researchers to create cell lines carrying mutations that cause specific diseases, thereby facilitating the study of disease mechanisms and the development of potential gene therapies.
Drug Screening: Cas9 Stable Cell Line - THP1 can be used in high-throughput drug screening, enabling rapid testing of thousands of compounds to identify those that affect specific gene function. This helps speed up the discovery and development of new drugs.
Functional genomics studies: The Cas9 stable cell line - THP1 can be used to perform functional genomics studies. Such cells can be used to generate libraries of cells in which a different gene has been knocked out in each cell. High-throughput sequencing can then be used to identify which genes are critical for cell survival or other functions.
Immunotherapy research: Because THP1 cells are originally derived from a human monocytic cell line, they can also be used in immunotherapy research and development. This includes enhancing the ability of immune cells to fight cancer or developing antibodies that can specifically target cancer cells.
For research use only. Not intended for any clinical use.
