Cas9 Stable Cell Line - Jurkat
Cat.No. : CSC-RO0179 Host Cell: Jurkat
Size: >1x10^6 cells/vial Validation: T7 Endonuclease I assay
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Cell Line Information
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Datasheet
| Cat. No. | CSC-RO0179 |
| Product Type | Cas9 overexpression stable cell line |
| Introduction | Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR). |
| Cell Line Information | Jurkat-Cas9 cell line is engineered to stably overexpress Cas9 nuclease. The Cas9 nuclease in Jurkat-Cas9 cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, Jurkat-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc. It is also an ideal cell line model for sgRNA screening and validation, either individually or in pools. |
| Target Gene | Cas9 |
| Host Cell | Jurkat |
| Applications | 1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation |
| Quality Control | 1) T7E1 assay 2) Mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6 cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Jurkat cells, derived from the peripheral blood of a 14-year-old patient with T-cell acute lymphoblastic leukemia (T-ALL), are a well-known human T lymphocyte cell line commonly used in cell biology research, particularly in cancer research and immune system disorder investigations. Jurkat cells produce interleukin-2 and are used in research involving cancer's sensitivity to drugs and radiation. Jurkat cells are commonly used in HIV research because they express CD4 receptors on their cell membranes. The CD4 receptor is the primary receptor used by HIV to enter host cells. Because Jurkat cells express this receptor, they can be infected by HIV, making them a useful model for studying HIV's interaction with human T cells, the virus's primary target in the body. The use of Jurkat cells in studies of HIV activation and the life cycle of HIV infection has greatly contributed to the understanding of viral interactions with human cells and helped to identify potential targets for antiretroviral therapy.
Cas9 Stable Cell Line-Jurkat is a cell line that has been genetically engineered to stably express Cas9 endonuclease. The enzyme is a key component of the CRISPR/Cas9 system, a widely used genome-editing tool that allows genes to be added, deleted or changed within an organism's cells. The use of stable cell lines ensures consistent Cas9 expression, allowing for more reliable and efficient gene editing compared to traditional transient transfection methods. Additionally, this stable cell line eliminates the need for continuous transfection of cells with Cas9, increasing the efficiency and cost savings of genome editing experiments.
Cas9 stable cell line-Jurkat is widely used in various scientific and biomedical fields due to its powerful gene editing capabilities. Here are some of its main applications:
Gene Editing: The Cas9 protein allows scientists to precisely edit parts of the genome by removing, adding, or changing parts of the DNA sequence.
Biomedical Research: Cas9 Stable Cell Lines - Jurkat are frequently used in biomedical research, particularly in the study of human T lymphocyte function and diseases such as cancer and autoimmune diseases.
Drug Development: Gene manipulation using Cas9-stable cell lines Jurkat could help develop new therapeutics.
Disease Modeling: Cas9 Stable Cell Line-Jurkat can be used to model disease in the laboratory, helping researchers understand disease mechanisms and develop potential treatments.
Immunotherapy Research: Jurkat cells are used in immunology to study T cell receptor signaling and to develop new immunotherapy approaches.
For research use only. Not intended for any clinical use.
