Cas9 Stable Cell Line - Panc 10.05
Cat.No. : CSC-RO0221 Host Cell: Panc 10.05
Size: >1x10^6 cells/vial Validation: T7 Endonuclease I assay
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Cell Line Information
Safety and Packaging
| Cat. No. | CSC-RO0221 |
| Product Type | Cas9 overexpression stable cell line |
| Introduction | Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR). |
| Cell Line Information | Panc 10.05-Cas9 cell line is engineered to stably overexpress Cas9 nuclease. The Cas9 nuclease in Panc 10.05-Cas9 cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, Panc 10.05-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc. It is also an ideal cell line model for sgRNA screening and validation, either individually or in pools. |
| Target Gene | Cas9 |
| Host Cell | Panc 10.05 |
| Applications | 1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation |
| Quality Control | 1) T7E1 assay 2) Mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6 cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Species | Homo sapiens (Human) |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Case Study
Pancreatic cancer represents a challenging malignancy with complex genetic landscapes characterized by numerous somatic mutations. The researchers demonstrated an innovative CRISPR-Cas9 strategy targeting specific somatic mutations with protospacer adjacent motifs (PAMs), enabling potentially selective cancer cell elimination. Their approach involved comprehensive whole genome sequencing, identifying multiple tumor-specific genomic targets, and developing a precise gene-editing mechanism capable of achieving 69-99% selective cell death in pancreatic cancer cell lines.
Figure 1. The researchers leveraged Panc10.05 and TS0111 cell lines to validate their CRISPR-Cas9 strategy, systematically testing multiple sgRNA targets to assess selective cancer cell killing efficiency and specificity through comprehensive genomic and cellular analyses. (Teh SSK, et al., 2024)
Creative Biogene's Panc 10.05 Cas9 Stable Cell Line offers researchers a robust platform for advanced cancer studies. Our meticulously developed cell line provides a reliable tool for investigating targeted gene editing strategies.
For research use only. Not intended for any clinical use.
