Cas9 Stable Cell Line - PC3
Cat.No. : CSC-RO1013 Host Cell: PC3
Size: >1x10^6 cells/vial Validation: T7 Endonuclease I assay
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Cell Line Information
Safety and Packaging
| Cat. No. | CSC-RO1013 |
| Product Type | Cas9 overexpression stable cell line |
| Introduction | Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR). |
| Cell Line Information | PC3-Cas9 cell line is engineered to stably overexpress Cas9 nuclease. The Cas9 nuclease in PC3-Cas9 cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, PC3-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc. It is also an ideal cell line model for sgRNA screening and validation, either individually or in pools. |
| Target Gene | Cas9 |
| Host Cell | PC3 |
| Applications | 1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation |
| Quality Control | 1) T7E1 assay 2) Mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6 cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Species | Homo sapiens (Human) |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Case Study
Metastasis is a key factor in the progression of prostate cancer (PCa), particularly when cancer cells spread to bone tissue. Understanding the molecular mechanisms that drive this process is critical for developing new treatments. The researchers focused on MAP4K4, identifying it as a crucial gene in PCa metastasis. They demonstrated that deleting MAP4K4 or inhibiting its kinase, HGK, impaired the migration and clonogenic abilities of metastatic PCa cells. Their findings revealed that HGK depletion alters cell spreading and focal adhesion, disrupting the cell's response to TNF-α stimulation and chemoattractants. Additionally, high levels of HGK correlated with poor prognosis in PCa patients, positioning it as a potential biomarker for aggressive cancer phenotypes. The researchers utilized the PC3 Cas9 stable cell line to perform targeted gene knockdowns.
Figure 1. The researchers investigated the impact of HGK down-regulation on prostate cancer cell migration and adhesion. Using the PC3 Cas9 stable cell line, they employed Western blotting to confirm HGK depletion and performed migration and wound healing assays. Their results showed that HGK silencing reduced migration and enhanced adhesion in PCa cells. (Garcia-Garcia S, et al., 2021)
For research use only. Not intended for any clinical use.
