CRISPR Mouse for Immunodeficiency Disease Modeling    

Acquired immunodeficiency syndrome (AIDS) virus is a retro RNA virus, designated as human immunodeficiency virus (HIV), which mainly attacks and massively destroys CD4+ T lymphocytes, the most important part of the human immune system, causing a significant reduction in the body's cellular immune function and ultimately leading to Gene editing technologies such as ZFN and CRISPR/Cas9 may remove or destroy the HIV integrated genome or HIV-infected cells from multiple HIV reservoirs, which provides technical support for a complete cure of AIDS in the future.

Humanized mouse models from immunodeficient mice have been the primary tool for studying human infectious viruses such as HIV. Over the past few decades, mice have become the model of choice for medical experiments. Techniques for manipulating the mouse genome can be used to assess the role of individual genes in a variety of biological processes. The use of genetically engineered mouse models may play an important role in revealing the function of a large number of new genes.

Fig. 1 Humanized mice for HIV research. (Victor Garcia J., 2016)

Solution

CRISPR/Cas9 Platform^CB generates different kinds of immunodeficient mouse models by embryonic co-microinjection of Cas9 mRNA and multiple sgRNAs targeting mice B2m, Il2rg, Prf1, Prkdc, and Rag1. We can implement multiple gene modifications, fragment deletions, and double knockouts of genes on the same chromosome to generate different kinds of immunodeficient mouse models with different heritable gene modifications. We create multiple mouse models that mimic immunodeficiency diseases by turning on or off specific genes in specific cell types and/or at specific times. We can also effectively disrupt cellular genes and integrated HIV-1 proviral DNA required for HIV-1 infection via CRISPR/Cas9. In addition, we can assess the role of various genes in the pathogenesis of immunodeficiency diseases, and we can provide very rich information in mapping functional pathways against these diseases.

What Can We Help You?

• Study the pathogenesis of immunodeficiency diseases
• Provide insights into immune system development and function
• Analysis of HIV replication, assessment of HIV limiting factors, characterization of HIV prevention strategies, and evaluation of new treatment options

Related Services

• Conditional Knockout Mouse
• Conventional Knockout Mouse
• Point Mutation Mouse
• CRISPR/Cas9 Knockin Mouse
• Rosa26 Knockin Mouse

Related Products

• CRISPR/Cas9 Kits
• Cas9 Protein
• Cas9 mRNA
• Cas9 Related Plasmids
• Pre-made Cas9 Virus Particles

CRISPR/Cas9 Platform^CB offers a variety of gene editing services, including knockout and knock-in mouse models. Our gene editing systems are continuously optimized for higher efficiency, faster gene editing, and more feasible complex targeting designs. In addition, we offer a best-in-class quality control program using Southern blot to ensure the successful delivery of each gene targeting project. If you are interested in our services, contact us now to request your custom gene editing mice.