Bcr Gene Editing    

Bcr (breakpoint cluster region protein), also named renal carcinoma antigen NY-REN-26, has serine/threonine kinase activity and is a GTPase activating protein of p21Rac. Bcr contains a unique structure for two opposite regulatory activities of small GTP-binding proteins and is associated with the signal transduction of Rho GTPases and the regulation of Rac1 activity. Its C-terminus is a GTPase activating protein (GAP) domain that stimulates GTP hydrolysis by Rac1, Rac2, and CDC42, accelerates the intrinsic rate of GTP hydrolysis of Rac1 or CDC42, resulting in downregulation of the active GTP binding form. The central Dbl homology (DH) domain acts to regulate the GTPase CDC42, RhoA and Rac1 guanine nucleotide exchange factor (GEF), promoting the conversion of CDC42, RhoA, and Rac1 from GDP binding to GTP binding. The amino terminus contains intrinsic kinase activity.

Studies have shown that Bcr can regulate macrophage function by regulating Rac1 activity and play an important negative regulatory role in neuronal Rac1 activity. Moreover, the BCR-ABL oncoprotein oligomerization domain found at the N-terminus of BCR is essential for the carcinogenicity of the BCR-ABL fusion protein. BCR-related diseases include leukemia, chronic granulocytes and leukemia, and acute lymphocytes. At present, it is unclear about the normal Bcr function and it is still unclear. Further researches are needed to help understand the relationship between Bcr and disease development.

Bcr Gene Editing Service

CRISPR/Cas9 Platform^CB, one of the leading gene editing biotechnology companies, is committed to building efficient systems and procedures to meet the needs and timelines of clients working in the CRISPR/Cas9 gene editing. We have deep gene editing knowledge and extensive experience in experimental operation and data processing and provide comprehensive CRISPR/Cas9 gene editing services to help you effectively control target genes knockout/knockin/point mutation in cells or animals.

• Bcr Gene Editing Cell Line Generation

With advanced national-level labs, a team of professional scientists, and extensive experience with cell culture and CRISPR system application, CRISPR/Cas9 Platform^CB has successfully implemented Bcr CRISPR/Cas9 gene edit in both easy-to-transfect cell lines and hard-to-transfect cells. Tell us your needs, we will offer you full-length Bcr gene editing services from strategy design to final stable cells. Our Bcr gene editing cell line generation services include:

✧ SgRNA design and synthesis
✧ Transfect the cell line you interest
✧ Select the high expression cell and sort monoclonal cell
✧ Validate the knockout/knockin/point mutation of Bcr by PCR and sequencing
✧ Produce cryogenic preserved vials of stable cells and a final report

Typically, we develop CRISPR-mediated gene editing cell lines, including HEK239T, Hela, HepG2, U87, but we can use other cell lines according to your requirements.

Host cell line: Ba/F3, CHO, MDA-MB-453, MDA-MB-231NIH3T3, T47D, Neuro2a, MCF7, RKO, K562, RAW264.7, etc.

• Bcr Gene Editing Animal Model Generation

CRISPR/Cas9 Platform^CB also has extensive experience in incorporating CRISPR-Cas9 technology into animal models, which have been fully recognized by our clients. According to your projects’ needs, we offer a one-stop-shop Bcr CRISPR/Cas9 gene editing animal service and guarantee at least 2 founders or 3 F1 animals with shorter turnaround time and lower price. Our Bcr gene editing animal model generation services include:

➢ Bcr gene conventional knockout animals
➢ Bcr gene conditional knockout animals
➢ Bcr point mutation animals
➢ Bcr knockin animals

Alternative species: mouse, rat, rabbit, zebrafish, C. elegans, etc.

CRISPR/Cas9 Platform^CB is devoted to providing the best gene editing services to accelerate the achievement of your research goals. Our one-stop service covers from strategy design to final stable cell lines or F1 animals. In addition, customized service has been provided to meet your special requirements. We guarantee our clients the most reliable and efficient research services to best match your research goals and protect your science investment. There is no doubt that CRISPR/Cas9 Platform^CB will be your best partner to support your research. If you have any project need CRISPR system, don’t hesitate to contact us.

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