Mouse B2m Knockout Cell Line-NIH 3T3
Cat.No. : CSC-RT2624
Host Cell: NIH3T3 Target Gene: B2m
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2624 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of mouse B2M using CRISPR/Cas9. |
| Target Gene | B2m |
| Host Cell | NIH3T3 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Mouse |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Beta-2-microglobulin (B2M) is an important component of the major histocompatibility complex (MHC) class I molecules, which are present in almost all nucleated cells. The B2M protein is encoded by the B2M gene located on chromosome 15 in humans. The main function of B2M is to participate in the presentation of endogenous antigens to T cells, especially CD8+ cytotoxic T cells. This process is essential for the immune system's ability to recognize and eliminate cells infected by viruses and other pathogens, as well as to detect and destroy cancer cells. In addition to its role in immune surveillance, B2M is associated with other molecules such as CD1, MR1, and the neonatal Fc receptor (FcRn), each of which plays a role in immune responses and cellular interactions.
Elevated levels of B2M may indicate the presence of chronic inflammation, certain infections, and a range of malignancies, including multiple myeloma and certain types of lymphoma. In patients receiving long-term hemodialysis, B2M can aggregate into amyloid fibrils, resulting in dialysis-associated amyloidosis, which is characterized by deposits in joint spaces and other tissues. Some viruses, such as cytomegalovirus (CMV), produce proteins that bind to B2M, preventing the assembly and trafficking of MHC class I molecules to the cell surface. This evasion strategy allows these viruses to avoid detection by the immune system and highlights the important role of B2M in viral immune evasion mechanisms.
The mouse B2m knockout cell line - NIH 3T3 is a valuable tool in biomedical research. Here are the main applications of this cell line:
Immunology Research: Since β2m is a component of MHC class I molecules, these KO cells help researchers understand how the loss of β2m affects antigen presentation and T cell interactions.
Cancer Research: By using NIH 3T3 cells without β2m, scientists can study how β2m affects tumor growth, metastasis, and immune evasion. This cell line helps identify new therapeutic targets and test the efficacy of immunotherapies designed to enhance the immune response to tumors.
Autoimmune Disease Modeling: B2m KO NIH 3T3 cells can be used to model autoimmune diseases where MHC class I molecules play a role. Researchers can study how the loss of β2m affects the development and progression of diseases such as type 1 diabetes and multiple sclerosis.
Drug Screening and Development: This cell line can be used as a platform for testing new drugs and therapeutic compounds that target pathways involving β2m and MHC class I molecules. By observing the effects on KO cells, researchers can gather data on the efficacy and toxicity of new drugs in a controlled environment.
For research use only. Not intended for any clinical use.
