Mouse B2m Knockout Cell Line-Hepa1-6
Cat.No. : CSC-RT2789
Host Cell: Hepa1-6 Target Gene: B2m
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2789 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of mouse B2m using CRISPR/Cas9. |
| Target Gene | B2m |
| Host Cell | Hepa1-6 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The beta-2-microglobulin (B2m) gene encodes a component of the class I major histocompatibility complex (MHC I) molecule, which is present in all nucleated cells. B2m is located on chromosome 15 in humans and plays a key role in the immune system, particularly in the presentation of peptide antigens to T cells. One of the main functions of B2m is to stabilize the tertiary structure of MHC class I molecules, thereby ensuring their proper expression on the cell surface. Without this stabilization, MHC class I molecules are misfolded and cannot be effectively transported to the cell surface. This results in decreased immune surveillance and increased susceptibility to viral infections and cancer.
Mutations or deletions in the B2m gene have been associated with various diseases. For example, a deficiency in B2m causes heavy chain deposition disease (HCDD), a type of amyloidosis in which monoclonal immunoglobulin heavy chains are deposited in tissues, leading to organ dysfunction. In addition, the absence of functional B2m impairs the presentation of endogenous antigens, making it easier for pathogens such as viruses and certain cancers to evade immunity. In addition to its role in the immune system, B2m has been studied as a potential biomarker for a variety of diseases. Elevated levels of B2m in serum or urine may indicate renal dysfunction, chronic inflammation, or lymphoproliferative disorders.
Applications of Mouse B2m Knockout Cell Line (Hepa1-6)
Immunology Research: Mouse B2m Knockout Cell Line (Hepa1-6) cells can serve as a model to dissect immune responses and antigen presentation mechanisms, aiding in the development of treatments for autoimmune diseases and immune deficiencies.
Cancer Research: The use of Mouse B2m Knockout Cell Line (Hepa1-6) cells can provide insights into tumor immunology, metastatic processes, and the interface between cancer and the immune system. They are also used to screen and develop novel anticancer drugs that modulate immune responses.
Vaccine Development: By utilizing this cell line, researchers can better understand how the absence of β2-microglobulin affects the presentation of viral antigens. This understanding is critical for developing vaccines that can elicit a strong immune response, especially when considering personalized vaccine strategies for cancer and infectious diseases.
Drug Screening and Development: β2-microglobulin knockout cell lines are ideal systems for high-throughput screening of drugs. Because β2-microglobulin has been implicated in the progression of a variety of diseases, compounds that affect its pathways can be efficiently tested in these cells, accelerating the discovery of potential therapeutics.
For research use only. Not intended for any clinical use.
