Human EGFR Knockout Cell Line-PC3
Cat.No. : CSC-RT2770
Host Cell: PC3 Target Gene: EGFR
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2770 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human EGFR using CRISPR/Cas9. |
| Target Gene | EGFR |
| Host Cell | PC3 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The epidermal growth factor receptor (EGFR) gene, also known as ERBB1 and HER1 in humans, encodes a transmembrane protein that belongs to the ErbB receptor family. The EGFR protein plays a key role in regulating cell growth, survival, proliferation, and differentiation. When bound to its specific ligands, such as epidermal growth factor (EGF) and transforming growth factor-α (TGF-α), EGFR undergoes a conformational change that leads to its dimerization and activation via autophosphorylation on key tyrosine residues. This activation triggers multiple downstream signaling pathways, including the MAPK, Akt, and JNK pathways, which are essential for cell division and proliferation.
EGFR is essential for a variety of normal cellular processes, including the development of epithelial tissues and the maintenance of the integrity of the skin barrier. It has also been shown to be involved in the immune response of human skin, highlighting its importance in normal physiology and disease states. However, mutations and overexpression of the EGFR gene have been implicated in the pathogenesis of several cancers. These cancers include lung adenocarcinoma, glioblastoma, and epithelial tumors of the head and neck. In these cancers, alterations in EGFR lead to its persistent activation, which results in uncontrolled cell proliferation and survival, promoting tumor growth and progression. For example, a specific mutation, called EGFRvIII, is frequently observed in glioblastoma and is associated with increased malignancy.
Applications of Human EGFR Knockout Cell Line-PC3:
1. Cancer Research: EGFR plays a key role in cell proliferation and survival. By knocking out EGFR in prostate cancer cell line PC3 cells, researchers can study the biological and biochemical pathways involved in cancer growth and progression without the influence of EGFR signaling.
2. Drug Screening and Development: Targeting EGFR is a common strategy in cancer treatment. By using EGFR knockout PC3 cells, researchers can test the efficacy and specificity of potential drug candidates.
3. Biomarker Discovery: Knockout cell lines can help identify alternative biomarkers for cancer diagnosis and prognosis. By comparing the cell behavior and molecular profiles of knockout and wild-type cells, new biomarkers independent of EGFR signaling can be discovered.
4. Resistance Mechanisms: EGFR inhibitors often face the problem of drug resistance. Studying EGFR knockout PC3 cells can reveal alternative pathways and resistance mechanisms, guiding the development of combination therapies to overcome resistance.
For research use only. Not intended for any clinical use.
