Our Services
CRISPR Off-Target Effects Analysis
Custom C. elegans Model
Custom Cell Lines Services
Custom Drosophila Model
Custom Rabbit Model
Custom Rat Model
Custom Zebrafish Model
Gene Knockout Mouse Generation
Genome Editing in Plants
Knockin Cell Line Generation
Knockout Cell Line Generation
Microbial Genome Editing Services
sgRNA Design and Expression,
Cas9 Expression
A typical sgRNA contains 98 nucleotides (including a 20-nt target sequence) and functions as a guide in the Cas9/sgRNA nuclease complex. To improve Cas9 expression in plants, most modified Cas9 genes for genome editing have also been optimized with usage bias codons.
Delivery of the Cas9 and sgRNA
Expression Cassettes
In CRISPR/Cas9 system, there are two critical parts: sgRNA sequence and Cas9 sequence. These two sequence could be cloned into separated plasmids or combined into one plasmids.
Considering the limited utility of protoplast system, it’s also available to transfect preassembled complexes of purified Cas9 protein and synthesized sgRNA.
Analyze editing efficiency
The genotype of the resultant mutants must be determined before carrying out further studies.
- Using Reporter Genes to Verify Targeted Mutations
- Using Endonucleases to Verify Targeted Mutations
- Using PAGE to Verify Targeted Mutations
- Using High-Resolution Melting to Verify Targeted Mutations
- Using High-Throughput Sequencing/ Sanger Sequencing to Determine Targeted Mutations
Why Choose Us
We Offer Best Services & Solutions
Creative Biogene provides reliable and professional gene editing services and products by CRISPR/Cas9. At Creative Biogene, we are dedicated to meet the needs of clients with high quality products and services in a reasonable price. To date, we have succeeded in lots of custom cell line generation projects and developing more than two thousand knockout cell lines.