CRISPR Library Screening

As a leader of CRISPR product manufacturer, Creative Biogene CRISPR/Cas9 Platform offers a comprehensive CRISPR library screening service and sgRNA library products for clients. With leading-edge technology and excellent platform, our experienced scientists are expert in CRISPR library screening. In order to fulfill different requirements, CRISPR/Cas9 Platform^CB has developed various gRNA libraries, such as human genome-wide knockout library, mouse genome-wide knockout library, pathway-related gRNA library, CRISPRa library and CRISPRi library. Custom gRNA library is welcome as well.

CRISPR library screening is a high-throughput screening method of important molecular targets. An efficient CRISPR screen starts with a well-designed sgRNA library targeting the genes or loci of interest. The sgRNA library presents in two forms, pool and array. Either pooled library or arrayed library conducted, it has good performance in screening. SgRNA are designed based on sequence and then synthesis followed by cloning to vector or transcribing into RNA for transfection in cell system. The transfected cells are detected for pre-set parameters and the results are analyzed at final. For different purpose, there are different detection assay.

Fig1. Workflow of CRISPR library screening

CRISPR/Cas9 Platform^CB provides a CRISPR library screening service for different purposes, including but not limited:

Drug targets screening
Drug-sensitive gene selection
Multi-gene drug targets screening
Phenotypic screening

Reference:

Peng, J., et al. (2015) High-throughput screens in mammalian cells using the CRISPR-Cas9 system. FEBS J 282, 2089-2096
Sun, W., et al. (2018) Genome-wide CRISPR screen reveals SGOL1 as a druggable target of sorafenib-treated hepatocellular carcinoma. Lab Invest 98, 734-744
Agrotis, A., et al. (2015) A new age in functional genomics using CRISPR/Cas9 in arrayed library screening. Front Genet 6, 300

For research use only. Not intended for any clinical use.

Inquiry