Human PIEZO1 Knockout Cell Line-A549
Cat.No. : CSC-RT2796
Host Cell: A549 Target Gene: PIEZO1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2796 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human PIEZO1 using CRISPR/Cas9. |
| Target Gene | PIEZO1 |
| Host Cell | A549 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
PIEZO1 is a gene that encodes a protein called piezoelectric mechanosensitive ion channel component 1. PIEZO1 protein is found in many tissues throughout the body, such as blood vessels, kidneys, lungs, and bladder. Structurally, PIEZO1 forms a three-bladed propeller-like structure, as revealed by crystallographic studies. This unique structure is essential for its function, enabling the channel to respond to mechanical stress by changing its conformation. Functionally, PIEZO1 acts as a pore-forming subunit of a mechanosensitive ion channel, allowing the influx of cations, such as calcium ions, upon mechanical stimulation. This gating mechanism has profound consequences for cell physiology, affecting processes as diverse as ion transport across membranes, regulation of cell adhesion, integrin activation, and cellular responses to mechanical stimulation.
PIEZO1 mutations are associated with various genetic diseases. For example, hereditary polycythemia (HX), a rare genetic disease characterized by dehydration of red blood cells. In patients with this disease, certain mutations in PIEZO1 cause overactive ion channels, which results in an imbalance in ion transport, leading to reduced cell size and increased red blood cell fragility. Clinical manifestations of HX include anemia, jaundice, and an increased risk of gallstones. In addition to inherited red blood cell disorders, studies have implicated PIEZO1 in other disorders, such as lymphatic dysplasia. Mutations in PIEZO1 can lead to dysfunction of lymphatic valves, which can result in improper lymphatic flow regulation and lymphedema. Additionally, there is emerging evidence that PIEZO1 may play a role in cancer metastasis, as its mechanosensitive properties affect how cancer cells behave as they migrate and invade new tissues.
Applications of Human PIEZO1 Knockout Cell Line-A549
Mechanotransduction Research: The PIEZO1 gene is known to encode a mechanosensitive ion channel involved in converting mechanical stimuli into electrochemical signals. By utilizing the Human PIEZO1 Knockout Cell Line-A549, researchers can study the specific role of PIEZO1 in mechanotransduction pathways, helping to elucidate its function and regulatory mechanisms in human cells.
Disease Modeling: PIEZO1 has been implicated in various diseases, including polycythemia vera and dehydrating hereditary stomatitis (DHS). The PIEZO1 knockout model provides an important tool to understand how loss of PIEZO1 function causes these diseases at the cellular level, which may lead to the development of new therapeutic strategies.
Cancer Research: PIEZO1 is often involved in tumor progression and metastasis. The Human PIEZO1 Knockout Cell Line-A549 is an important model for studying how PIEZO1 affects cancer cell migration, invasion, and response to mechanical changes in the tumor microenvironment.
Drug Screening: This knockout cell line can be used for high-throughput screening of drugs targeting mechanosensitive pathways. It enables researchers to identify and characterize new pharmacological agents that modulate PIEZO1 activity or compensate for its loss, potentially leading to new treatments for diseases associated with mechanotransduction dysfunction.
Cell Physiology Studies: PIEZO1 channels are integral to a variety of cellular physiological processes, including cell volume regulation, calcium signaling, and stress responses. By comparing cellular processes in PIEZO1 knockout cells and wild-type A549 cells, researchers can gain a deeper understanding of the physiological role of PIEZO1 and its contribution to overall cellular function.
For research use only. Not intended for any clinical use.
