Human LRRK2 Knockout Cell Line-Jurkat
Cat.No. : CSC-RT2780
Host Cell: Jurkat Target Gene: LRRK2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2780 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of LRRK2 using CRISPR/Cas9. |
| Target Gene | LRRK2 |
| Host Cell | Jurkat |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The LRRK2 gene, also known as leucine-rich repeat kinase 2, is located on chromosome 12 (12q12) and is responsible for the production of the LRRK2 protein, a large multidomain protein that belongs to the ROCO protein family. The LRRK2 protein has multiple functional domains, including the leucine-rich repeats, the Roc (Ras of complexin) GTPase domain, the C-terminal of the Roc (COR) domain, and the kinase domain. The protein is involved in various cellular pathways, such as vesicle trafficking, mitochondrial function, and autophagy. These pathways are essential for maintaining cellular health and function, especially in neurons.
Mutations in the LRRK2 gene are the most common genetic cause of Parkinson's disease (PD). The most prominent mutation is G2019S, in which the glycine at position 2019 is replaced by a serine. This and other mutations in the LRRK2 gene can lead to abnormal kinase activity, which can cause neuronal damage and degeneration. In addition to Parkinson's disease, LRRK2 has also been implicated in Crohn's disease and leprosy, suggesting a role in immune response and inflammation. Currently, inhibitors of LRRK2 kinase activity are being developed and tested in clinical trials, offering hope for new treatments for PD and other diseases that may be associated with LRRK2 dysfunction.
Human LRRK2 Knockout Cell Line - Jurkat is a valuable tool for biomedical research. Here are the main applications of this cell line:
Neuroscience Research: The LRRK2 gene is closely associated with neurological diseases, especially Parkinson's disease. By using the LRRK2 knockout Jurkat cell line, researchers can study the cellular and molecular mechanisms of neuronal dysfunction and degeneration, gaining insights into potential therapeutic targets for treating or managing Parkinson's disease.
Drug Screening and Development: This cell line allows for high-throughput screening of chemical compounds. This application is critical for the discovery of new drugs that can mimic or inhibit LRRK2 activity, providing a pathway to develop new treatments for diseases where LRRK2 plays an important role.
Immunology Research: LRRK2 is known to play a role in the regulation of immune responses. The Jurkat cell line is a T lymphocyte model and is an excellent candidate cell for studying the interaction of LRRK2 with immune cell signaling pathways.
Cancer Research: Aberrant expression of LRRK2 has been associated with various cancers. Using the human LRRK2 knockout Jurkat cell line, researchers can study the role of LRRK2 in cancer cell proliferation, survival, and metastasis. This can identify new biomarkers for cancer diagnostics and potential therapeutic targets.
Pathway Analysis: LRRK2 is part of several important signaling pathways, and the knockout cell line allows for detailed pathway analysis to understand how the deletion affects cell signaling and homeostasis.
For research use only. Not intended for any clinical use.
