Human LRRK2 Knockout Cell Line-HeLa
Cat.No. : CSC-RT1698
Host Cell: HeLa Target Gene: LRRK2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT1698 |
| Cell Line Information | A stable cell line with a homozygous knockout of human LRRK2 using CRISPR/Cas9. |
| Target Gene | LRRK2 |
| Host Cell | HeLa |
| Shipping | 10^6 cells/tube |
| Storage | Liquid nitrogen |
| Species | Human |
| Gene ID | 120892 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Leucine-rich repeat kinase 2 (LRRK2) is a multidomain enzyme encoded by the LRRK2 gene, located on human chromosome 12. It is primarily expressed in various tissues, including brain, kidney, lung, and immune cells. LRRK2 is a member of the ROCO protein family, characterized by the presence of a leucine-rich repeat (LRR), a Ras-like GTPase domain (Roc), a C-terminal (COR) domain of Roc, a kinase domain, and multiple other protein-protein interaction domains. LRRK2 protein plays a crucial role in cellular processes such as apoptosis, autophagy, and vesicle trafficking.
Mutations in the LRRK2 gene have been identified as one of the most common genetic causes of familial and sporadic Parkinson's disease (PD). Significant mutations include G2019S, R1441C/G, and Y1699C, all of which alter kinase activity and lead to neuronal toxicity and death. In particular, the G2019S mutation increases LRRK2 kinase activity, which has been implicated in pathological processes such as alpha-synuclein aggregation, a hallmark of Parkinson's disease.
The Human LRRK2 Knockout Cell Line - HeLa provides a versatile platform for numerous applications in biomedical research. Here are some of the key uses:
Parkinson's Disease Research: LRRK2 mutations are the leading cause of familial Parkinson's disease. This cell line lacking LRRK2 allows researchers to study the cellular and molecular mechanisms behind the disease, which can help identify potential therapeutic targets.
Drug Screening and Development: LRRK2 Knockout HeLa cells can be used to screen for compounds that modulate the activity of pathways affected by LRRK2. This is particularly useful for testing new drugs designed to treat Parkinson's disease and other neurodegenerative diseases.
Gene Function Studies: By comparing knockout cell lines to wild-type cells, scientists can gain insight into the normal biological function of LRRK2, exploring its role in cellular processes such as vesicle trafficking, cytoskeletal dynamics, and autophagy.
Pathway Analysis: These cells can be used to dissect signaling pathways in which LRRK2 participates. This includes the MAPK pathway, intracellular trafficking, and inflammatory responses, helping to gain a broader understanding of cell biology.
Protein Interaction Studies: In the absence of LRRK2, knockout cells can help identify proteins and molecular interactions that are dependent on LRRK2.
Disease Modeling: In addition to Parkinson's disease, LRRK2 has been implicated in several other diseases, such as Crohn's disease and certain types of cancer. This cell line can be used to model these diseases in vitro, helping to better understand how LRRK2 deficiency affects cellular physiology.
For research use only. Not intended for any clinical use.
