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Knockout Cell Lysate 
The use of CRISPR/Cas9 gene editing technology to generate knockout cell lines and cell lysates ensures the development of useful tools to validate antibody specificity and elucidate gene function. Knockout cell lines and cell lysates are widely recognized as the best negative controls, demonstrating the specificity of antibodies to target targets and ensuring reproducibility quickly and confidently.
With many years of experience in gene editing, CRISPR/Cas9 PlatformCB is able to pride you with 100% knockout cell lysate to meet your specific research needs. Our knockout cell lysates is made from double knockout cell lines that provide perfect negative control for western blot to verify antibody specificity or for functional assays. Each knockout cell lysate carries a wild type control that matches the cell line. To facilitate transport and protein, the products are provided in the form of lyophilized protein.
What is available for each KO Lysate product?
- KO cell lysate
- Parental cell lysate
How to verify the knockout cell line?
Each KO cell line carries a mutation at both alleles of the locus of interest.
Validation is performed at genomic levels by PCR and sequencing using PCR primers strategically designed around the target site or inserted fragments.
What are the applications of KO cell lysate?
- The direct use is to serve as a negative control for western blotting and ultimately verify the specificity of the antibody.
- Functional assays
- In vitro assays
In addition, if you can't find the gene you're interested in, or the cell line of interest, check out our custom cell line services. There is no doubt that CRISPR/Cas9 PlatformCB will be your best partner to support your research.