Description
This product is a circRNA encoding spCas9 protein. This product will produce high expression level of spCas9 protein and should be used together with sgRNA for gene editing.
Applications
Gene editing (KO/KI/Point Mutation cell line generation)
Quality Control
• RNase R resistance test of circular RNA.
• Integrity analysis
• Sanger sequencing of the back-splicing junction.
Storage
Store at -70°C or below. Avoid repeated freeze/thaw cycles. Aliquot if necessary using RNase-free equipment, reagents, pipet tips, tubes, and containers.