Mouse Tfap2a Knockout Cell Line-NIH 3T3
Cat.No. : CSC-RT2617
Host Cell: NIH3T3 Target Gene: Tfap2a
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2617 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of mouse Tfap2a using CRISPR/Cas9. |
| Target Gene | Tfap2a |
| Host Cell | NIH3T3 |
| Size Form | 1 vial (10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Mouse |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
Transcription factor AP-2 alpha (Tfap2a) is a key regulatory protein that plays a vital role in the development and differentiation of various tissues and organs. It belongs to the AP-2 family of transcription factors, which are characterized by their ability to bind to specific DNA sequences and regulate the expression of target genes. One of the main functions of Tfap2a is in embryonic development, where it is expressed in multiple tissues, including the neural crest, eyes, and limbs. In the neural crest, Tfap2a is essential for the migration, survival, and differentiation of neural crest cells. The absence or mutation of Tfap2a leads to severe developmental defects, including craniofacial abnormalities, which emphasizes its importance in early development.
Tfap2a also plays an important role during eye development. It regulates the expression of genes involved in the development of the lens and retina, ensuring the normal formation and function of these structures. Mice lacking Tfap2a exhibit ocular abnormalities. In addition, Tfap2a plays an important role in skin biology. It is involved in the differentiation of keratinocytes and the formation of the epidermis. In addition to its developmental role, Tfap2a has been implicated in disease. Aberrant expression of Tfap2a has been observed in various cancers, suggesting that it may play a role in tumorigenesis. For example, overexpression of Tfap2a has been associated with the progression of melanoma and breast cancer. This makes Tfap2a not only a key player in developmental processes but also a potential target for therapeutic intervention in cancer.
The Mouse Tfap2a Knockout Cell Line (NIH 3T3) serves as a powerful tool in various aspects of biomedical research, particularly for studying the roles and mechanisms associated with the Tfap2a gene. Below are some of its key applications:
Gene Function Analysis: Studying the Mouse Tfap2a Knockout Cell Line in NIH 3T3 cells allows researchers to investigate the specific roles of the Tfap2a gene. By observing phenotypic changes and genetic pathways affected by the knockout, scientists can gain insights into gene function and regulation.
Developmental Biology: Tfap2a plays a crucial role in developmental processes. Utilizing NIH 3T3 cells with a Tfap2a knockout provides a model to observe and understand developmental mechanisms, including cell differentiation, proliferation, and tissue formation.
Cancer Research: Tfap2a is implicated in various cancers. By creating a Tfap2a knockout in NIH 3T3 cells, researchers can model tumor biology, study the gene’s role in oncogenesis, cancer progression, metastasis, and potentially identify new therapeutic targets.
Signal Transduction Studies: The Tfap2a gene is involved in various signaling pathways. NIH 3T3 Tfap2a knockout cell lines can be used to dissect these pathways, offering insights into cellular responses to different stimuli and uncovering potential points of intervention in disease contexts.
Drug Screening and Development: NIH 3T3 cells with Tfap2a knocked out serve as a powerful platform for high-throughput drug screening. They allow for the identification of compounds that might correct or mitigate the impacts of Tfap2a deficiency, facilitating the development of novel therapeutics.
For research use only. Not intended for any clinical use.
