Mouse Lats2 Knokcout Cell Line-4T1
Cat.No. : CSC-RT2616
Host Cell: 4T1 Target Gene: Lats2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2616 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of mouse Lats2 using CRISPR/Cas9. |
| Target Gene | Lats2 |
| Host Cell | 4T1 |
| Size Form | 1 vial (10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Mouse |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
LATS2, also known as large tumor suppressor kinase 2, is a serine/threonine protein kinase that plays a vital role in various cellular processes. One of the most critical functions of LATS2 is its role in the Hippo signaling pathway, a highly conserved regulatory network that controls organ size by regulating cell growth, apoptosis, and stem cell renewal. In this pathway, LATS2 and its homolog LATS1 phosphorylate and inactivate downstream effectors such as YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif). By doing so, LATS2 prevents these effectors from translocating into the nucleus, where they would otherwise promote the expression of genes that drive cell proliferation and inhibit apoptosis. Thus, LATS2 acts as a tumor suppressor, helping to control cell growth and prevent carcinogenesis.
In addition to its role in the Hippo pathway, LATS2 is involved in several other cellular mechanisms. It plays a role in mitotic checkpoint control, ensuring that chromosomes are properly aligned and segregated, thereby maintaining genomic stability. LATS2 interacts with other key regulatory proteins, including p53, and has been shown to stabilize this well-known tumor suppressor, promoting its activity in response to cellular stress and DNA damage. Mutations and alterations in LATS2 expression have been observed in various cancers, including breast cancer, gliomas, and hematological malignancies, underscoring its importance as a tumor suppressor. Therefore, understanding the molecular mechanisms by which LATS2 operates is critical for developing therapeutic strategies against cancer and other diseases characterized by dysregulated cell proliferation and apoptosis.
The Mouse Lats2 Knokcout Cell Line-4T1 serves as an invaluable tool in research settings for a variety of applications:
Tumorigenesis Studies: Investigate the role of the Lats2 gene in tumorigenesis, including how loss of function affects tumor growth, progression, and metastasis.
Drug Testing: Screen potential anti-cancer drugs and therapies. By understanding how Lats2 gene knockout influences drug sensitivity and resistance, more effective cancer treatments can be developed.
Gene Function: Elucidate the gene function and its pathways by comparing the knockout cell line with the wild-type to understand the molecular mechanisms in cancer development and cell cycle regulation.
Signal Transduction Studies: By using the Mouse Lats2 Knockout Cell Line-4T1, researchers can study the Hippo signaling pathway, in which LATS2 plays a critical role.
Genetic Interaction Analysis: This cell line serves as a tool to investigate genetic interactions and dependencies. By introducing other genetic modifications or treatments, researchers can analyze how the absence of LATS2 affects cellular responses.
Mouse Model Studies: The 4T1 cell line is often used to create mouse models of breast cancer. The Lats2 knockout version can be implanted into mice to study tumor growth and metastasis in vivo.
For research use only. Not intended for any clinical use.
