Mouse Cd47 Knockout Cell Line-MC38
Cat.No. : CSC-RT2698
Host Cell: MC38 Target Gene: Cd47
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2698 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of mouse Cd47 using CRISPR/Cas9. |
| Target Gene | Cd47 |
| Host Cell | MC38 |
| Size Form | 1 vial (10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Mouse |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
CD47 is a transmembrane protein that is widely expressed on various cells, including those in the immune system, making it a key player in cell interactions and immune responses. CD47, also known as integrin-associated protein (IAP), belongs to the immunoglobulin superfamily and is involved in different physiological and pathological processes. The protein interacts with signal regulatory protein α (SIRPα) on the surface of macrophages, inhibiting their ability to engulf and destroy cells. This interaction is essential for maintaining self-tolerance, preventing the immune system from attacking its own cells. Cancer cells are known to exploit this mechanism by overexpressing CD47, thereby escaping immune surveillance and destruction. This has made CD47 an interesting target for cancer research, leading to the development of therapeutic agents designed to block the CD47-SIRPα interaction, thereby enhancing the ability of the immune system to target and eliminate tumor cells.
In addition to its anti-phagocytic role, CD47 is involved in regulating other cellular processes such as apoptosis, proliferation, migration, and adhesion. Studies have shown that it is involved in regulating integrin signaling, which is essential for cell motility and stability. This makes CD47 an integral part of various physiological processes, including development, homeostasis, and wound healing. Recent studies have also implicated CD47 in diseases other than cancer. For example, its dysregulation has been observed in autoimmune diseases, cardiovascular diseases, and neurodegenerative diseases.
The mouse CD47 knockout cell line - MC38 is a valuable tool for biomedical research, primarily used to explore the role of CD47 in cancer biology and immunotherapy. CD47 is often referred to as a "don't eat me" signal that acts as an inhibitory signal for macrophage-mediated phagocytosis. Here are some key applications of this knockout cell line:
Cancer Immunotherapy Research: The MC38 cell line with the Cd47 gene knocked out is widely used in cancer immunotherapy research. By knocking out Cd47, researchers can study how the absence of this signal affects tumor growth and response to immune checkpoint inhibitors. This can reveal new therapeutic targets and enhance the efficacy of existing cancer treatments.
Drug Development: In drug research, MC38 Cd47 knockout cells can be used to screen for novel drugs that target the CD47-SIRPα interaction pathway. By understanding how these drugs affect cancer cells that lack Cd47, researchers can develop more effective therapies that enhance the immune system's ability to destroy cancer cells. This also helps evaluate the safety and efficacy of potential new drugs.
Tumor Immune Evasion Mechanisms: Studying Cd47 knockout MC38 cells allows researchers to delve into the mechanisms by which tumors evade the immune system. Because CD47 plays a critical role in protecting cells from immune system attack, its absence can provide insight into alternative immune evasion pathways and help identify additional potential therapeutic targets within the tumor microenvironment.
Biomarker Studies: The MC38 Cd47 knockout cell line is a valuable tool for biomarker discovery and validation. By comparing differences in immune responses and tumor progression between wild-type and Cd47 knockout cells, researchers can identify biomarkers that predict response to immunotherapy.
For research use only. Not intended for any clinical use.
