Human WRN Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2775
Host Cell: HEK293T Target Gene: WRN
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2775 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human WRN using CRISPR/Cas9. |
| Target Gene | WRN |
| Host Cell | HEK293T |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The WRN gene, also known as Werner syndrome RecQ-like helicase (RECQL2, RECQL3, RECQ3), is a key human gene involved in maintaining genome integrity. It is located on chromosome 8 and encodes Werner protein, which plays an important role in DNA repair and replication. Werner protein primarily functions as a helicase, an enzyme that unwinds and separates double-stranded DNA. In addition, it acts as an exonuclease, trimming damaged DNA 3' ends. These dual functions enable Werner protein to resolve complex DNA structures and ensure accurate DNA replication and repair.
Mutations in the WRN gene cause Werner syndrome, a disease characterized by premature aging and increased risk of cancer. More than 60 different mutations are known to cause Werner syndrome, which usually result in a truncated and non-functional Werner protein. This defective protein is unable to localize to the cell nucleus and perform its critical role, resulting in disruptions in the DNA repair, replication, and transcription processes. As a result, cells accumulate DNA damage over time, accelerating the aging process and predisposing individuals to a variety of age-related diseases.
Cancer Research: Werner syndrome is linked to premature aging and increased cancer susceptibility. By using WRN knockout HEK293T cells, researchers can study the mechanisms by which WRN deficiency contributes to genomic instability and cancer development.
Aging Studies: WRN is involved in DNA repair and maintaining genomic stability. The knockout cell line is invaluable for understanding the molecular pathways of aging and identifying new strategies to combat age-related diseases.
DNA Repair Mechanisms: Since WRN plays a substantial role in DNA repair, the WRN knockout HEK293T cells are used to investigate the specific pathways and mechanisms impacted by WRN deficiency. This can aid in developing therapies for diseases caused by defective DNA repair mechanisms.
Drug Testing and Development: The WRN knockout cell line can be used to screen for drugs that might enhance the repair of DNA or protect against DNA damage. This can be particularly useful for developing treatments for cancer and age-related diseases.
Model for Genetic Disorders: HEK293T cells with WRN knockout can serve as a model for Werner syndrome, a genetic disorder characterized by premature aging. This enables better understanding of the disease and helps in developing potential therapies.
For research use only. Not intended for any clinical use.
