Human TREM2 Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2756
Host Cell: HEK293T Target Gene: TREM2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2756 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human TREM2 using CRISPR/Cas9. |
| Target Gene | TREM2 |
| Host Cell | HEK293T |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The TREM2 gene, also known as triggering receptor expressed on myeloid cells 2, is an essential gene in the human body that encodes a protein involved in immune responses. The gene is located on chromosome 6 and is primarily expressed in microglia. Microglia play a crucial role in maintaining homeostasis in the central nervous system (CNS) and mediating immune responses within the CNS. The protein encoded by the TREM2 gene functions as a cell surface receptor and is involved in several key processes, including phagocytosis of apoptotic cells, inflammatory responses, and clearance of amyloid deposits.
One of the most important aspects of TREM2 is its association with neurodegenerative diseases. Mutations in the TREM2 gene are associated with an increased risk of Alzheimer's disease, frontotemporal dementia, and Nasu-Hakola disease. These mutations disrupt the normal function of TREM2, leading to impaired clearance of amyloid plaques, a hallmark of Alzheimer's disease. This impaired clearance leads to increased inflammation and neuronal damage, which contributes to the progression of neurodegenerative diseases.
Applications of Human TREM2 Knockout Cell Line-HEK293T
Neurodegenerative Disease Research: TREM2 is a receptor expressed in microglia that plays a crucial role in regulating the brain's immune response. By using TREM2 knockout cells, researchers can elucidate how the loss of this receptor affects microglial function and leads to neurodegeneration.
Drug Screening and Development: This knockout cell line is a valuable tool for screening potential therapeutic compounds targeting TREM2-related pathways. By observing cellular responses upon TREM2 loss, scientists can identify drugs that may compensate for the loss of TREM2 function or modulate its signaling pathways, thereby facilitating the development of new treatments for neurodegenerative diseases.
Immune Response Studies: TREM2 has been implicated in the regulation of macrophage and microglial activity. Human TREM2 knockout cell line-HEK293T enables researchers to study the effects of TREM2 deficiency on immune responses. This can provide insight into how TREM2 affects inflammation and immune cell activation, which is critical to understanding a variety of inflammatory and autoimmune diseases.
Cellular and Molecular Pathway Elucidation: Researchers can study how TREM2 interacts with other proteins and receptors, its downstream signaling effects, and its role in cellular processes such as phagocytosis and cell survival.
For research use only. Not intended for any clinical use.
