Human TP53 Knockout Cell Line-MV-4-11
Cat.No. : CSC-RT2704
Host Cell: MV4-11 Target Gene: TP53
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2704 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human TP53 using CRISPR/Cas9. |
| Target Gene | TP53 |
| Host Cell | MV4-11 |
| Size Form | 1 vial (10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The TP53 gene, also known as "tumor protein p53", is one of the most intensively studied and important genes in cancer research because of its key role in maintaining genome stability and acting as a tumor suppressor. The TP53 gene is located on the short arm of chromosome 17 (17p13.1) and spans approximately 20 kilobases, including several highly conserved regions. The protein product, p53, functions primarily by binding to DNA and regulating the expression of target genes. This regulation is essential for initiating DNA repair processes when damage occurs, halting the cell cycle to provide time for repair, and triggering apoptosis when necessary to prevent the proliferation of cells with DNA errors.
TP53 gene mutations are the most common genetic alterations in human cancer, present in more than 50% of cases. These mutations often result in the loss of functional p53 protein, leading to uncontrolled cell division and tumor development. The gene can be affected by point mutations, insertions, deletions, and other variations that affect coding sequences and regulatory regions. The therapeutic potential of targeting the p53 pathway in cancer treatment is enormous. Strategies include restoring wild-type p53 function in mutant cancers or reactivating p53 in the presence of oncogenic inhibitors. In addition, the role of p53 in cellular stress responses extends its relevance beyond oncology to areas such as aging and the treatment of other diseases involving genomic instability.
The TP53 gene encodes the tumor protein p53, which plays a key role in regulating the cell cycle and preventing cancer formation. TP53 knockout cell lines, such as the MV-4-11 cell line, are valuable tools in a variety of research areas. Here are some key applications:
Cancer Research: The TP53 knockout MV-4-11 cell line is essential for studying the role of the TP53 gene in cancer development. The TP53 gene is known as the "guardian of the genome" for its role in preventing genomic mutations. Studies involving TP53 knockout cells help understand how mutations in this gene lead to cancer progression.
Drug Screening and Development: By comparing the effects of drugs on TP53 knockout cells and wild-type cells, researchers can identify compounds that specifically target pathways affected by loss of TP53 function.
Gene Function Studies: TP53 knockout cell lines allow scientists to study the specific functions of the TP53 gene in cell cycle regulation, apoptosis, and DNA repair mechanisms.
Tumor Suppression Mechanism Studies: Researchers can use these cell lines to elucidate the molecular mechanisms by which TP53 suppresses tumor formation. This can reveal potential therapeutic targets in TP53-regulated pathways.
Pathway Analysis: Utilizing TP53 knockout cell lines facilitates the analysis of cellular pathways that are altered due to TP53 loss. This can include pathways involved in cell proliferation, senescence, and response to DNA damage, providing a comprehensive understanding of the role of TP53 in maintaining cellular homeostasis.
For research use only. Not intended for any clinical use.
