Human TNFRSF1A Knockout Cell Line-HEK293
Cat.No. : CSC-RT2700
Host Cell: HEK293 Target Gene: TNFRSF1A
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2700 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human TNFRSF1A using CRISPR/Cas9. |
| Target Gene | TNFRSF1A |
| Host Cell | HEK293 |
| Size Form | 1 vial (10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The TNFRSF1A gene encodes a protein that belongs to the tumor necrosis factor receptor superfamily. This protein is commonly referred to as tumor necrosis factor receptor 1 (TNFR1) or CD120a. TNFR1 is a ubiquitously expressed membrane receptor that plays a crucial role in mediating various cellular responses, including apoptosis, inflammation, and cell proliferation. Upon binding to TNFα, TNFR1 can activate several intracellular signaling pathways that regulate the transcription factor NF-κB, which is essential for regulating immune responses, cell survival, and proliferation. In addition, TNFR1 can induce apoptosis through the death domain in its intracellular portion, which interacts with other proteins such as TRADD, FADD, and RIPK1 to propagate the death signal.
Germline mutations in the TNFRSF1A gene can lead to a disease called tumor necrosis factor receptor-associated periodic syndrome (TRAPS), which is characterized by recurrent fever and inflammation. Impaired receptor clearance is thought to be the underlying mechanism of this disease. These mutations have also been associated with increased susceptibility to autoimmune diseases such as multiple sclerosis. Elevated serum TNFR1 levels have been observed in psychiatric disorders such as schizophrenia and bipolar disorder and are associated with more severe psychotic symptoms and cognitive impairment.
Human TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily Member 1A) knockout cell lines, specifically derived from HEK293 cells, are valuable tools in biomedical research. Here are some of their key applications:
Inflammatory Disease Research: The TNFRSF1A gene encodes TNF receptor 1, which plays a crucial role in mediating inflammatory responses. Knocking out TNFRSF1A in HEK293 cells allows researchers to study the molecular mechanisms behind inflammatory diseases such as rheumatoid arthritis, Crohn's disease, and psoriasis. By observing how the loss of TNFRSF1A affects the response of cells to TNF-alpha, new therapeutic targets can be identified.
Cancer Research: TNFRSF1A is also involved in cancer cell signaling pathways. Knockout cell lines can help researchers study the effects of TNFRSF1A loss on cell proliferation, apoptosis, and tumor development. This can provide a better understanding of the role of TNFRSF1A in cancer progression and may facilitate the development of novel cancer therapies.
Drug Screening and Development: Using the TNFRSF1A knockout cell line, scientists can screen and develop new drugs designed to modulate the TNF/TNFRSF1A signaling pathway. The cell line can be used as a model to test the efficacy and toxicity of candidate drugs.
Signaling Transduction Studies: TNFRSF1A knockout in HEK293 cells provides a unique model for studying cellular signaling pathways. Researchers can observe how the removal of TNFRSF1A affects downstream signaling events, helping to map cellular networks and identify key regulatory nodes.
Genetic Studies and Functional Genomics: The cell line can be used for genetic studies to elucidate the functional role of TNFRSF1A. By comparing gene expression profiles between wild-type and knockout cells, scientists can identify genes and pathways that are directly or indirectly regulated by TNFRSF1A.
For research use only. Not intended for any clinical use.
