Human STAT1 Knockout Cell Line-HEK293
Cat.No. : CSC-RT2745
Host Cell: HEK293 Target Gene: STAT1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2745 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human STAT1 using CRISPR/Cas9. |
| Target Gene | STAT1 |
| Host Cell | HEK293 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The STAT1 gene encodes the signal transducer and activator of transcription 1 protein, a key member of the STAT protein family. This gene plays a crucial role in mediating various cellular responses to cytokines and growth factors. When these ligands bind to their corresponding receptors, the receptor-associated kinase phosphorylates the STAT1 protein, which subsequently forms homo- or hetero-dimers. These dimers translocate to the nucleus and act as transcriptional activators to regulate gene expression. STAT1 is activated by a variety of ligands, including interferon-α, interferon-γ, EGF, PDGF, and IL6. This activation is essential for cell viability, immune responses to viral, fungal, and mycobacterial pathogens, and a variety of other cellular processes.
STAT1 mutations are associated with several immunodeficiency diseases, such as immunodeficiency 31A, 31B, and 31C. These mutations can result in either loss- or gain-of-function, each resulting in different clinical manifestations. Loss-of-function mutations hamper the immune system's ability to respond to infection, leading to increased susceptibility to viral and bacterial pathogens. In contrast, gain-of-function mutations are often associated with chronic mucocutaneous candidiasis (CMC), leading to an overstimulated immune response, resulting in recurrent infections, often involving Candida species, and are frequently associated with other autoimmune diseases.
The human STAT1 knockout cell line - HEK293 provides a valuable model for biological and medical research. The following are the main applications of this cell line:
Signal Transduction Studies: STAT1 protein plays a key role in the cellular response to cytokines and growth factors through the JAK-STAT pathway. Using STAT1 knockout HEK293 cells, researchers can study signaling mechanisms and pathway alterations in the absence of STAT1, gaining insight into the role of STAT1 in cellular processes.
Cancer Research and Drug Development: Given STAT1's involvement in tumor suppression and immune response regulation, STAT1 knockout cells are essential for understanding cancer cell behavior. These cells help determine the effects of STAT1 loss on tumorigenesis and test new anticancer drugs that target STAT-related pathways.
Infectious Disease and Immunology Research: STAT1 is essential in mediating the cellular response to viral and bacterial infections. Researchers use STAT1 knockout HEK293 cells to study how STAT1 loss affects immune responses, pathogen replication, and the effectiveness of antiviral treatments.
Gene Expression and Regulation: By examining changes in gene expression in STAT1 knockout cells, scientists can identify STAT1-dependent genes and regulatory networks. This helps map the transcriptional landscape affected by STAT1 and understand its role in gene regulation under various physiological and pathological conditions.
For research use only. Not intended for any clinical use.
