Human SLC40A1 Knockout Cell Line-HEK293
Cat.No. : CSC-RT2708
Host Cell: HEK293 Target Gene: SLC40A1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2708 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human SLC40A1 using CRISPR/Cas9. |
| Target Gene | SLC40A1 |
| Host Cell | HEK293 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The SLC40A1 gene, also known as solute carrier family 40 member 1, encodes a protein that is essential for iron metabolism in humans and various other organisms. The gene is specifically responsible for the production of ferroportin, a transmembrane protein that is an important iron exporter in the body. Ferroportin facilitates the transport of iron across cell membranes, thereby ensuring that iron absorbed from the diet is effectively distributed throughout the body. Hepcidin binds to ferroportin and induces its internalization and degradation, thereby controlling the amount of iron released into the blood. This interaction ensures that whole-body iron homeostasis is maintained—when iron levels are high, hepcidin rises to reduce ferroportin activity, and when iron levels are low, hepcidin falls to increase iron export.
Mutations in the SLC40A1 gene have been associated with various forms of inherited iron overload disorders, most notably a hereditary form of hemochromatosis called ferroportinopathy or type 4 hemochromatosis. More than 37 different mutations in this gene have been associated with this disease, which is characterized by the body's inability to regulate iron levels, leading to excess iron accumulation in tissues and organs. Individuals with ferroportinopathy typically develop symptoms in adulthood, and the disease is inherited in an autosomal dominant pattern.
Applications of Human SLC40A1 Knockout Cell Line-HEK293
1. Functional Genomics Studies
SLC40A1 knockout cell lines can be used to study the functional role of the SLC40A1 gene in various cellular processes. By comparing the behavior of knockout cells with wild-type cells, researchers can gain insight into the physiological and pathological roles of this gene.
2. Disease Modeling
SLC40A1 is implicated in iron homeostasis and related diseases. Using knockout cell lines, scientists can model diseases such as hemochromatosis and determine how the lack of SLC40A1 leads to disease progression.
3. Drug Discovery and Development
Knockout cell lines are valuable tools for screening drugs targeting pathways affected by SLC40A1. This helps identify potential therapeutic compounds for the treatment of iron metabolism disorders and other related diseases.
4. Iron Metabolism Studies
Since the SLC40A1 gene is primarily involved in the transport of iron, knockout cell lines are an important model for studying the absorption, distribution, and regulation of iron at the cellular level. The researchers can explore how cells compensate for the absence of this gene and identify other key players in iron metabolism.
For research use only. Not intended for any clinical use.
