Human SDCBP Knockout Cell Line-Hela
Cat.No. : CSC-RT2720
Host Cell: Hela Target Gene: SDCBP
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2720 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human SDCBP using CRISPR/Cas9. |
| Target Gene | SDCBP |
| Host Cell | Hela |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
The SDCBP gene encodes a protein called syntenin-1, which is present in humans and plays a crucial role in cellular functions. One of the main functions of syntenin-1 involves organizing the cytoskeleton and membrane structure, which is essential for maintaining cell shape, stability, and intracellular trafficking. The interaction of this protein with adherens junctions and focal adhesions emphasizes its role in cell adhesion, which is essential for tissue integrity and signal transduction. In addition, syntenin-1 also affects the activation of transcription factors, affecting gene expression and cellular responses.
The SDCBP gene exhibits alternative splicing, resulting in multiple transcript variants encoding different isoforms of syntenin-1. Its expression has been found in various tissues, including the retinal pigment epithelium, visceral pleura, amniotic fluid, and skin. Notably, syntenin-1 interacts with several important proteins, such as EFNB1, GRIK1, GRIK2, Merlin, and TRAF6, among others. These interactions highlight its involvement in a wide range of signaling pathways and cellular mechanisms, such as positive regulation of transforming growth factor-β receptor signaling, intracellular signal transduction, and organization of the actin cytoskeleton.
The Human SDCBP Knockout Cell Line-Hela is a powerful tool to advance research in a variety of biomedical fields. The following are the main applications of this cell line:
1. Cancer Research: The Human SDCBP Knockout Cell Line-Hela provides a valuable model for studying the role of SDCBP (syndecan-binding protein) in cancer progression and metastasis. Since SDCBP is involved in cell adhesion, migration, and signaling pathways, knocking out this gene in Hela cells helps to elucidate its contribution to tumor biology.
2. Drug Screening: This knockout cell line can be used for high-throughput drug screening to identify potential therapeutic compounds that target pathways affected by SDCBP loss. By comparing the responses between wild-type and knockout cells, researchers can identify drugs that specifically modulate the relevant cellular mechanisms.
3. Signal Transduction Studies: SDCBP is known to interact with a variety of signaling molecules. Knockout cell lines serve as models for more precise studies of these signaling pathways, allowing scientists to understand how the loss of SDCBP affects signal transduction and downstream effects.
4. Gene Function Analysis: The human SDCBP knockout cell line-Hela is ideal for basic studies of gene function. Researchers can observe phenotypic changes due to SDCBP knockout, gaining insight into its physiological role and interactions with other cellular proteins.
5. Biomarker Discovery: By utilizing this cell line in proteomic and genomic studies, new biomarkers associated with the SDCBP pathway can be identified.
For research use only. Not intended for any clinical use.
