Human RIOX2 Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2754
Host Cell: HEK293T Target Gene: RIOX2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2754 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human RIOX2 using CRISPR/Cas9. |
| Target Gene | RIOX2 |
| Host Cell | HEK293T |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The RIOX2 gene, also known as ribosomal oxygenase 2, encodes a protein that plays a key role in multiple biological processes in humans. The gene is part of the human genome and is located on chromosome 3 at position 3q11.2. RIOX2 is involved in the oxidative demethylation of histones, specifically targeting lysines 4 and 36 on histone H3, a key modification in regulating gene expression. Its enzymatic activities include binding metal ions and acting as a histone demethylase, which is able to shape chromatin structure and gene transcriptional landscape. Functionally, RIOX2 plays both positive and negative roles in transcriptional regulation through various mechanisms. It interacts prominently with RNA polymerase II and functions as a transcriptional corepressor. RIOX2 is involved in histone demethylation, ribosome biogenesis, and chromatin remodeling - key processes necessary for regulating gene expression, protein synthesis, and cell proliferation.
The RIOX2 gene is expressed in multiple tissues, with prominent expression in the thyroid, skeletal muscle, and pancreas, and also during key developmental stages such as blastoderm, ectoderm, and primitive streak formation. Alterations in RIOX2 expression levels have been observed in various cancers, including lung and colon cancer, where its overexpression has been associated with tumor progression. The protein product of RIOX2 has been extensively studied for its interaction with the MYC oncogene, suggesting its involvement in cell proliferation and cancer biology. In addition, RIOX2 has potential roles beyond its enzymatic function. It is involved in transcriptional regulation and modulation of chromatin state.
The following are the main applications of Human RIOX2 Knockout Cell Line-HEK293T:
Gene Function Analysis: RIOX2 KO cell lines allow researchers to delve into the specific role of RIOX2 in cellular processes. By observing phenotypic changes and alterations in cell behavior when RIOX2 is missing, scientists can uncover its function and importance in various pathways.
Cancer Research: RIOX2 has been implicated in a variety of cancers due to its role in ribosome biogenesis and cell proliferation. Studying RIOX2 KO cell lines can help elucidate its contribution to cancer development and progression, which may help identify new cancer biomarkers or therapeutic targets.
Ribosome Biogenesis Studies: Because RIOX2 is involved in ribosomal RNA maturation, KO cell lines are an excellent model to study the mechanisms of ribosome biogenesis and the effects of ribosome dysregulation on cellular physiology.
Drug Screening and Development: RIOX2 KO cell lines can be used to screen compounds that specifically target pathways affected by RIOX2 loss. This is particularly useful for developing targeted therapies for diseases where RIOX2 plays a key role.
Epigenetic Studies: RIOX2 is involved in histone modifications, affecting gene expression and chromatin structure. Using RIOX2 KO cell lines, researchers can study the downstream effects of RIOX2 loss on the epigenetic landscape and determine the resulting changes in gene expression patterns.
For research use only. Not intended for any clinical use.
